Tryptone Soy Broth (Soybean Casein Digest Medium EP/USP/BP/JP) (Dehydrated)

Thermo Scientific™ Oxoid™ Tryptone Soy Broth (TSB) is a highly nutritious general-purpose medium suitable for the cultivation of bacteria and fungi. With its inclusion in various international pharmacopoeia standards (EP/USP/BP/JP), TSB is versatile and widely used for sterility testing, microbial enumeration, and enrichment in pharmaceutical, clinical, and food microbiology applications.

Key Features:

1. Nutrient-Rich Composition:
   • Includes pancreatic digest of casein and soya peptone, providing essential nutrients for fastidious organisms.
2. Pharmacopoeia Compliance:
   • Formulated to meet the requirements of EP, USP, BP, and JP standards.
3. Versatile Applications:
   • Supports aerobic and anaerobic cultivation, blood culture, selective media preparation, and sterility testing.
4. Wide Range of Use Cases:
   • Suitable for sterility testing, microbial enumeration, and enrichment of specific organisms in food, pharmaceutical, and clinical samples.

Typical Formula (per liter):

Preparation Instructions:

1. Reconstitution:
   • Dissolve 30 g of TSB powder in 1 liter of distilled water.
   • Mix well to dissolve completely.
2. Sterilization:
   • Autoclave at 121°C for 15 minutes.
3. Optional Additions:
   • Add sterile anticoagulants (e.g., sodium polyanethyl sulphonate or sodium citrate) for blood cultures.

Applications:

1. Aerobic and Anaerobic Cultivation:
   • Cultivation of a wide variety of bacteria and fungi, including fastidious organisms.
2. Sterility Testing:
   • Detection of viable microorganisms post-sterilization (as per USP, EP, BP, JP).
3. Blood Culture:
   • Enhanced growth promotion for isolating organisms from blood or other body fluids.
4. Selective Media Preparation:
   • Enrichment of Staphylococcus aureus or E. coli O157 using selective agents.
5. Resuscitation of Spores:
   • Effective in resuscitating heated spores of Geobacillus stearothermophilus.

Specifications:

Quality Control:

Non-Pharmaceutical Testing:

Pharmaceutical Testing:

1. Sterility Test:
   • Incubate at 20–25°C for 14 days; no growth indicates sterility.
2. Growth Promotion Test:

Advantages:

Precautions:

• Avoid prolonged exposure to light for prepared medium.
• Perform sterility tests in strict aseptic conditions.

References:

1. United States Pharmacopeia (USP 30) (2008).
2. Compendium of Methods for the Microbiological Examination of Foods (4th Ed.), APHA.
3. British Pharmacopoeia (BP, 2004).
4. European Pharmacopoeia (EP, 6.1 Edition, 2008).
5. Japanese Pharmacopoeia (JP, 15th Edition, 2006).

Thermo Scientific™ Oxoid™ TSB is an essential, versatile medium for reliable microbial cultivation, sterility testing, and enrichment, meeting international pharmacopoeia standards.