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OriGene One-Wash™ Lentivirus Titer Kit, HIV-1 p24 ELISA
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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Assay Kits | Forms | Kit with Various components |
| Product Brand | Origene | ||
| Product Grade | Molecular Biology | ||
Rapid Quantification of Lentiviral Titer via HIV-1 p24 Core Antigen Detection
The One-Wash Lentivirus Titer Kit (HIV-1 p24 ELISA) is a high-performance immunoassay designed for the quantitative measurement of HIV-1 p24 antigen in lentiviral particles. Utilizing a 96-well plate format pre-coated with anti-HIV-1 p24 antibodies, this kit allows for sensitive, rapid, and reproducible quantification of lentivirus preparations in just one wash step, streamlining workflow and reducing assay time.
This kit is ideal for researchers conducting lentiviral vector production, gene delivery, and transduction optimization in cell and gene therapy research.
Key Features & Benefits
- ✅ One-Wash Protocol – Streamlined ELISA workflow with fewer steps and reduced hands-on time
- ✅ Quantitative HIV-1 p24 Detection – Measure lentiviral titer via p24 core protein
- ✅ High Sensitivity & Specificity – Detect low levels of lentivirus with strong signal-to-noise ratio
- ✅ Validated Components – Each reagent QC-tested for reliable performance
- ✅ Complete Kit Format – Includes all necessary reagents for 96-well plate assays
- ✅ Stable & Ready-to-Use – Shelf-stable for up to 6 months at 4 °C
Kit Components
| Component | Volume/Format |
|---|---|
| HIV-1 p24 Antibody Coated 96-Well Plate | 1 plate (8×12 strips) |
| Recombinant HIV-1 p24 Standard | 100 µL |
| Biotinylated HIV-1 p24 Detection Antibody | 12 mL |
| HRP Conjugated Streptavidin-Peroxidase | 12 mL |
| Lysis Buffer | 5 mL |
| 20x Plate Wash Buffer | 60 mL |
| TMB Substrate | 12 mL |
| TMB Stop Solution (1 N HCl) | 12 mL |
Product Specifications
| Attribute | Details |
|---|---|
| Kit Type | HIV-1 p24 ELISA Lentivirus Titer Kit |
| Detection Method | Colorimetric ELISA (TMB, HRP-based) |
| Target Analyte | HIV-1 p24 capsid protein |
| Format | 96-well plate (strip format) |
| Sample Type | Lentiviral particle supernatants |
| Assay Time | ~2.5 hours |
| Storage Condition | 4 °C (upon receipt) |
| Stability | 6 months from date of receipt (if properly stored) |
| Shipping Condition | Blue Ice |
| Quality Control | Validated for lentiviral titer quantification |
Applications
🧬 Lentiviral Vector Production QC – Quantify viral titer in gene therapy manufacturing
🔬 Transduction Optimization – Standardize viral input across cell lines
🧪 Gene Delivery Research – Measure efficiency of lentiviral packaging and delivery
🏥 Preclinical Cell Therapy Studies – Monitor consistency and dosage of lentivirus formulations
The One-Wash Lentivirus Titer Kit (HIV-1 p24 ELISA) offers a fast, reliable, and user-friendly method to quantify lentivirus particles by detecting the HIV-1 p24 core antigen. With a simplified one-wash protocol and ready-to-use reagents, this kit is ideal for accelerating workflows in vector development, gene therapy research, and virology laboratories.