Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns

The Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns are rapid, ready-to-use centrifugal columns designed to efficiently remove non-conjugated fluorescent dyes, biotinylation reagents, reducing agents, and crosslinkers from labeled protein samples. Utilizing a 7K molecular weight cutoff (MWCO) proprietary resin, these columns allow for fast and effective small molecule removal while ensuring high recovery of proteins >7 kDa. The columns are optimized to clean up post-reaction protein labeling mixtures in less than 15 minutes without requiring any cumbersome column preparation or equilibration.

Key Features & Benefits

• Selective Cleanup of Protein Samples
  Removes unreacted dyes, biotin, crosslinkers, and reducing agents while retaining proteins >7 kDa.
• High Recovery Rates
  Proprietary low-binding resin preserves sample integrity and yields exceptional protein recovery, even from dilute samples (≥25 µg/mL).
• No Column Setup Required
  Ready-to-use format eliminates the need for resin hydration, column packing, or pre-equilibration.
• Fast Workflow
  Complete the cleanup process in under 15 minutes using standard benchtop centrifugation.
• Flexible Volumes & Formats
  Available in multiple sizes (0.5 mL, 2 mL, 5 mL, 10 mL) and in 96-well plate format for high-throughput applications.

Applications

• Post-Labeling Cleanup
  Ideal for purifying protein samples following labeling with fluorophores, biotin, or crosslinkers.
• Sample Prep for Spectroscopy or Quantitation
  Removes background signal from unreacted dyes to enable accurate dye-to-protein ratio measurements.
• Antibody and Enzyme Conjugate Purification
  Optimized for recovery of labeled IgG, enzymes, and other biomolecules following conjugation reactions.
• High-Throughput Protein Sample Cleanup
  96-well plate format supports automated processing of small-volume protein samples for screening assays or mass spectrometry.

Available Formats & Sample Volumes

Note: These columns are designed specifically for dye and reagent cleanup, not for general desalting or buffer exchange.

Specifications

The Thermo Scientific™ Zeba™ Dye and Biotin Removal Spin Columns offer an efficient, convenient, and high-recovery solution for post-labeling sample purification. With multiple size options and fast, low-effort protocols, these columns are an ideal choice for researchers conducting protein labeling, antibody conjugation, or fluorescence-based applications. Whether you're working with small-volume analytical assays or high-throughput workflows, Zeba columns ensure clean, interference-free results with minimal protein loss.