Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution

Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution is a high-purity, 10% (w/v) nonionic surfactant, specially formulated for reliable performance in ELISA, Western blotting, and immunoassays. Packaged under nitrogen gas in glass ampules or non-leaching HDPE bottles, this solution ensures long-term stability, eliminates peroxide accumulation, and prevents degradation.

Tween™ 20 is widely used in biochemical, molecular biology, and pharmaceutical research due to its low toxicity, excellent biocompatibility, and strong protein-stabilizing properties. This detergent plays a key role in reducing non-specific binding in assays, improving wash buffers, and solubilizing membrane proteins.

Key Features & Benefits

1. High-Purity 10% (w/v) Tween™ 20 Detergent Solution
   • Precisely formulated to ensure batch-to-batch consistency.
   • Stable nonionic surfactant suitable for sensitive biological applications.
2. Enhanced Stability & Purity
   • Packaged under nitrogen gas to prevent oxidation and degradation.
   • Contains <1.0 μeq/mL peroxides and carbonyls, ensuring high purity.
3. Ideal for Immunoassays & Molecular Biology
   • Optimized for ELISA, Western blot, and immunoassay wash buffers.
   • Reduces background noise in antibody-based assays.
4. Versatile Applications in Protein & Lipid Research
   • Effective in membrane protein extraction and stabilization.
   • Used in cell lysis, protein solubilization, and lipid-based assays.
5. Convenient & Ready-to-Use Format
   • Available in glass ampules or HDPE bottles for precise dispensing.
   • Easily diluted to desired working concentrations.

Product Specifications

Use Cases & Applications

1. Immunoassays & Antibody-Based Assays

• Blocking & Wash Buffer Additive in ELISA & Western Blot
  • Reduces non-specific binding to increase assay sensitivity.
  • Used in PBS and TBS buffers for consistent signal detection.
• Immunohistochemistry & Flow Cytometry Applications
  • Ensures optimal antigen-antibody interactions.
  • Minimizes background interference in fluorescence-based assays.

2. Protein Extraction & Stabilization

• Membrane Protein Solubilization & Isolation
  • Enhances protein extraction without denaturation.
  • Used in detergent-based cell lysis buffers.
• Enzyme Stabilization & Protein Purification
  • Prevents protein aggregation in chromatography & purification processes.
  • Ideal for enzymatic assays requiring nonionic detergents.

3. Molecular Biology & Genomics

• DNA & RNA Extraction Protocols
  • Used in nucleic acid purification buffers for cell lysis and protein removal.
  • Ensures RNA integrity in sensitive extraction protocols.
• PCR & qPCR Reaction Optimization
  • Enhances reaction efficiency by reducing surface tension.
  • Compatible with Taq polymerase-based amplification methods.

4. Cell Culture & Drug Delivery Research

• Detergent for Cell Membrane Permeabilization
  • Facilitates antibody staining and intracellular protein detection.
  • Commonly used in cell-based permeability assays.
• Surfactant in Liposome & Nanoparticle Formulations
  • Supports liposome stabilization in drug delivery systems.
  • Used in biocompatible emulsions and surfactant-stabilized formulations.

5. Industrial & Biotechnological Applications

• Emulsifier in Food & Beverage Processing
  • Acts as a stabilizer in emulsions and food formulations.
  • Ensures consistent texture and shelf-life improvement.
• Cosmetic & Personal Care Formulations
  • Used in lotions, shampoos, and skincare products for emulsion stability.
  • Provides mild surfactant properties in cleansing formulations.

Usage & Handling Instructions

Preparation & Dilution:

• For Immunoassay Buffers (ELISA, Western Blot):
  • Dilute Tween™ 20 to 0.05–0.1% (v/v) in PBS or TBS buffer.
• For Protein Solubilization & Lysis Buffers:
  • Use at 0.1–2% (w/v) depending on application.
• For Cell Culture & Permeabilization:
  • Use at 0.01–0.5% (w/v) for mild permeabilization.

Handling Precautions:

• Avoid prolonged exposure to air, as Tween™ 20 can degrade over time.
• Work in a well-ventilated environment when preparing solutions.
• Always use RNase/DNase-free conditions when working with nucleic acid extractions.

Storage Recommendations:

• Keep at room temperature in a dry, well-ventilated area.
• Protect from direct sunlight and heat to prevent degradation.
• Ensure cap is tightly sealed after each use to maintain reagent integrity.

Disposal Guidelines:

• Dispose of according to local biosafety and chemical waste disposal regulations.
• Avoid direct disposal into drains or water sources without proper neutralization.

Why Choose Thermo Scientific™ Tween™ 20 Surfact-Amps™?

✅ High-purity 10% Tween™ 20 solution for reproducible results

✅ Packaged under nitrogen gas to prevent peroxides and degradation

✅ Ready-to-use format for ELISA, Western blot, and cell culture applications

✅ Nonionic surfactant with excellent protein-stabilizing properties

✅ Compatible with immunoassays, molecular biology, and biopharmaceutical research

Thermo Scientific™ Tween™ 20 Surfact-Amps™ Detergent Solution is a reliable, high-purity surfactant designed for consistent and reproducible results in immunoassays, molecular biology, and industrial applications.