Thermo Scientific™ Tween 20 Washing/Blocking Buffer, 0.5% in PBS (10X)

Thermo Scientific™ Tween 20 Washing Buffer, 0.5% in PBS (10X) is a high-quality concentrated blocking and wash buffer solution developed for sensitive immunoassay applications. This ready-to-use PBS-based formulation contains 0.5% Tween 20, a gentle nonionic detergent that improves washing efficiency and helps reduce nonspecific antibody binding.

Widely used in Western blotting, ELISA, and other antibody-based detection protocols, this buffer supports consistent signal clarity by effectively removing unbound immunological reagents while preserving specific antigen–antibody interactions.

Supplied as a convenient 10X concentrate, it enables rapid preparation of working-strength wash or incubation buffer for routine laboratory use.

Key Features and Benefits

Effective Blocking and Washing Performance

Tween 20 acts as a surfactant that helps prevent nonspecific protein adsorption, reducing background and improving assay sensitivity.

Optimized for Immunoassays

Designed for use in:

• Western blot membrane washing
• ELISA microplate wash steps
• Immunoreagent incubation solutions
• General antibody-based detection workflows

Reduces Unspecific Background Signals

By limiting nonspecific antibody binding sites, the buffer enhances signal-to-noise ratio in immunodetection assays.

Convenient Concentrated Format

Delivered as a 10X stock solution, minimizing preparation time and ensuring reproducible dilution to 1X working concentration.

Ambient Storage Stability

Can be stored at ambient temperatures, simplifying laboratory handling and logistics.

Applications

Thermo Scientific™ Tween 20 Blocking Buffer Solution is commonly used for:

• Wash steps between immunoreactions
• Removal of unbound antibodies and proteins
• Preparation of labeled antibody incubation solutions
• Background reduction in Western blots and ELISAs
• Routine immunoassay buffer workflows

Product Specifications

Ordering Information

Thermo Scientific™ Tween 20 Washing/Blocking Buffer, 0.5% in PBS (10X), is a reliable and convenient solution for reducing nonspecific antibody binding and improving washing efficiency in Western blotting, ELISA, and other immunoassays. With its optimized detergent formulation, reproducible concentrate format, and ambient storage stability, it is an essential reagent for laboratories seeking consistent, low-background immunodetection results.