Thermo Scientific™ TBS with Tween® (TBST), 20X Solution

Thermo Scientific™ TBS with Tween® (TBST), 20X Solution is a high-quality, ultrapure Tris-buffered saline detergent buffer designed for sensitive molecular biology applications, particularly Western blotting, immunodetection, and protein analysis workflows. This ready-to-use concentrated solution contains Tris buffer, optimized salt composition, and 1.0% Tween 20, ensuring excellent washing efficiency while minimizing background noise in immunoassays.

Manufactured under strict contaminant control, this Molecular Biology Grade TBST is tested to be free of DNase and protease activity, making it ideal for high-sensitivity protein and nucleic acid laboratory protocols.

Key Features and Benefits

Optimized for Western Blotting Applications

TBST is widely used as a wash and dilution buffer in Western blot protocols to improve signal clarity and reduce nonspecific antibody binding.

Molecular Biology Grade Purity

Each batch is tested and certified for:

• DNase (endo): Pass
• DNase (exo): Pass
• Protease: Pass

This ensures reliable performance in sensitive downstream applications.

Ultrapure and Sterile Filtered

The solution is 0.2 μm filtered, supporting clean, contamination-free laboratory use.

Convenient Ready-to-Use Concentrate

Supplied as a 20X concentrate, allowing easy dilution to working strength (1X) for routine laboratory workflows.

Practical Packaging Design

Delivered in an upright carton with a convenient dispensing spout for safe handling and reduced waste.

Buffer Composition (20X Concentrate)

Prepared in high-purity deionized water and adjusted to pH 7.4:

• 500 mM Tris
• 60 mM KCl
• 2.8 M NaCl
• 1.0% Tween 20
• pH 7.4

Applications

Thermo Scientific™ TBST is suitable for:

• Western blot washing steps
• Antibody dilution buffer preparation
• ELISA wash buffer workflows
• Immunohistochemistry (IHC) protocols
• General protein blotting and detection methods
• Reducing nonspecific binding in immunoassays

Quality Specifications

Ordering Information

Thermo Scientific™ TBS with Tween® (TBST), 20X Solution provides a reliable, ultrapure, and contamination-tested buffer for Western blotting and immunoassay applications. With its optimized formulation, sterile filtration, and convenient concentrate format, it ensures consistent washing performance, reduced background, and high-quality results in demanding molecular biology laboratories.