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Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladder
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Protein Standards | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Four-color, prestained protein size standard for SDS-PAGE and western blotting
The Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladder contains 10 prestained proteins spanning 10 to 260 kDa, presented in four distinct colors (blue, orange, green, pink) for quick and accurate protein sizing during SDS-PAGE and western blotting.
Supplied in a ready-to-use format, this ladder requires no heating, reducing, or buffer addition before loading. It enables easy protein migration tracking during electrophoresis, as well as efficient transfer monitoring during western blotting. The multicolor banding pattern provides clear molecular weight reference points for accurate protein size estimation in both gels and blots.
Key Features & Benefits
- Wide Molecular Weight Range – 10 markers from 10 to 260 kDa for comprehensive protein size coverage.
- Four-Color Banding – Blue, orange, green, and pink bands for clear and easy band identification.
- Versatile Detection – Compatible with colorimetric, NIR fluorescence (700 nm), and RGB fluorescence (555 nm) imaging.
- Ready-to-Use – No heating, reducing, or buffer preparation needed before use.
- Broad Gel Compatibility – Works with Bolt™, Novex™, NuPAGE™, and other SDS-PAGE gel systems.
Applications
- Monitoring protein migration during SDS-PAGE
- Confirming protein transfer efficiency in western blotting
- Protein size estimation in gels and blots
- Multi-color reference for band identification in complex protein separations
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Protein Ladder |
| Product Line | Spectra™ |
| Number of Markers | 10 |
| Size Range | 10–260 kDa |
| Molecular Weights | 10, 15, 25, 35, 40, 50, 70, 100, 140, 260 kDa |
| Stain Type | 4 colors: Blue, Orange, Green, Pink |
| Detection Methods | Colorimetric, NIR fluorescence (700 nm), RGB fluorescence (555 nm) |
| Gel Compatibility | Bolt™ Bis-Tris Plus, Novex™ Tricine, Novex™ Tris-Glycine, NuPAGE™ Bis-Tris, NuPAGE™ Tris-Acetate, SDS-PAGE |
| Ready to Load | Yes |
| Unit Sizes | 26634 – 2 × 250 μL; 26634X4 – 8 × 250 μL; 26623 – 10 × 250 μL |
| Shipping Condition | Wet or dry ice |
| Storage Conditions | −20°C |
| Storage Buffer | 62.5 mM Tris-H₃PO₄ (pH 7.5), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 33% glycerol |
The Thermo Scientific™ Spectra™ Multicolor Broad Range Protein Ladder offers excellent versatility and clarity in protein sizing for SDS-PAGE and western blot workflows. With its four-color banding pattern, broad molecular weight range, and compatibility with multiple detection modes, it provides an accurate and convenient reference for protein migration and size estimation in research applications.
- Pack Size: 2 x 250 μL 8 x 250 μL 10 x 250 μL