Thermo Scientific™ Proteinase K, recombinant, PCR grade, 20 mg/mL

Thermo Scientific Proteinase K, Recombinant, PCR Grade is a highly effective serine protease used for protein digestion in nucleic acid preparations. It cleaves peptide bonds in the presence of detergents and works under a broad range of reaction conditions, making it ideal for use in various laboratory procedures, including genomic DNA isolation and cloning efficiency improvements.

Proteinase K is particularly useful for removing proteins and nucleases (such as DNases and RNases) during DNA and RNA extractions. The enzyme works efficiently even in the presence of detergents like SDS or urea, making it highly versatile for nucleic acid extractions from different sample types, including tissues, cultured cells, and mouse tails.

Key Features:

1. Recombinant Protein:
   • Produced recombinantly, ensuring high purity and consistent activity in every batch.
2. Broad-Spectrum Protease:
   • Efficiently cleaves aliphatic, aromatic, and hydrophobic amino acids, making it a versatile enzyme for protein degradation.
3. Active in Multiple Conditions:
   • Works in the presence of detergents such as SDS and urea, making it ideal for DNA and RNA extraction from cells, tissues, and cultured cell lines.
4. PCR Grade:
   • Certified for use in PCR applications, genomic DNA isolation, and improving cloning efficiency.
5. High Concentration:
   • The enzyme is provided at a concentration of >600 U/mL, ensuring high activity and effectiveness.
6. Storage and Stability:
   • Store at 4°C for short-term use or -20°C for long-term storage. The enzyme is stable over a wide pH range (4.0 to 12.5) and is most effective at a pH of 7.5 to 8.0.

Specifications:

Applications:

1. DNA/RNA Extraction:
   • Ideal for genomic DNA isolation from various sources such as mouse tail, tissues, and cultured cells. Proteinase K helps to eliminate proteins and nucleases to ensure high-quality nucleic acids for downstream applications.
2. Cloning and PCR:
   • Enhances cloning efficiency by removing contaminating proteins from PCR products, enabling successful transformation and gene cloning.
3. Enzyme Localization:
   • Used in enzyme localization studies by digesting proteins in tissue or cell samples while preserving the nucleic acid integrity.
4. Apoptosis Studies:
   • Proteinase K can be used to remove nucleases and study the effects of proteolysis during apoptosis.
5. Cellular and Biochemical Research:
   • Employed in cell biology and biochemical research for protein digestion and nucleic acid preparation.
6. Environmental Sample Preparation:
   • Utilized in environmental samples where proteins may be present in soil or water samples for molecular analysis.
7. DNA/RNA Integrity Studies:
   • Used to preserve DNA and RNA integrity during protein digestion steps, allowing for more accurate analysis and interpretation.

Related Products:

• Thermo Scientific™ DNA Extraction Kits
• Thermo Scientific™ RNA Isolation Kits
• Thermo Scientific™ Protein Lysis Buffers

Target Information:

Proteinase K is a serine protease from the fungus Engyodontium album. It is widely used in protein digestion and nucleic acid extraction protocols. The enzyme cleaves peptide bonds at the carboxylic sides of aliphatic, aromatic, and hydrophobic amino acids, making it highly efficient for cell lysis and nucleic acid purification.

Thermo Scientific™ Proteinase K, Recombinant, PCR Grade is an essential reagent for protein digestion and nucleic acid isolation. With its broad activity range, high purity, and robust performance in various applications, it is an invaluable tool for molecular biology, biochemistry, and cell biology research.