Thermo Scientific™ Pierce™ Polyacrylamide Spin Desalting Columns, 7K MWCO, 0.7 mL

The Thermo Scientific™ Pierce™ Polyacrylamide Spin Desalting Columns (7K MWCO, 0.7 mL) are ready-to-use, single-use centrifugal columns pre-packed with hydrophilic, non-reactive polyacrylamide resin. They are designed to enable rapid buffer exchange and desalting of proteins, peptides, and other macromolecules with molecular weights above 7,000 Da.

These columns offer superior separation performance without introducing low molecular weight contaminants, which are commonly associated with crosslinked dextran or agarose-based resins. The porous polyacrylamide resin has excellent flow properties and is highly resistant to microbial growth, chemical degradation, and temperature fluctuations.

Key Features & Benefits

• 7,000 Da Molecular Weight Cut-Off (MWCO)
  Efficiently separates peptides, proteins, and macromolecules larger than 7 kDa from salts, dyes, and other small molecules.
• High-Purity Polyacrylamide Resin
  Chemically stable and free from low molecular weight sugars or degradants.
• Non-Enzymatic, Microbe-Resistant Resin
  Resin does not degrade enzymatically and does not act as a microbial nutrient, extending storage life and performance consistency.
• Convenient Spin Format
  Enables quick sample processing (30–120 μL) via benchtop centrifugation, ideal for routine buffer exchange and sample cleanup.
• Broad pH and Chemical Compatibility
  Compatible with pH 2–10 at room temperature and autoclavable at pH 5.5–6.5.

Typical Applications

• Desalting protein or antibody solutions
• Removing salts, phenol, or other low-MW reagents from nucleic acids
• Cleanup after protein labeling, crosslinking, or radiolabeling
• Removing unreacted fluorescent dyes or biotin from conjugates
• Buffer exchange prior to enzyme assays, SDS-PAGE, or mass spectrometry
• Preparing purified proteins for downstream applications or storage

Specifications

The Pierce™ 7K MWCO Polyacrylamide Spin Desalting Columns offer a fast, reliable, and gentle approach for desalting and buffer exchange of small-volume biological samples. With minimal sample loss, no resin contamination, and compatibility across a broad pH and buffer range, these columns provide superior performance for both routine laboratory tasks and sensitive downstream workflows. Whether you're purifying labeled antibodies, removing salts from protein preparations, or preparing samples for mass spectrometry, the Pierce polyacrylamide desalting columns deliver dependable results with every spin.