Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate

Complete, rapid Western blot solution with Pierce™ ECL substrate – results in under an hour

The Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate streamlines the traditional Western blot workflow, reducing assay time from 4 hours to approximately 55 minutes without compromising sensitivity. Designed for blots probed with mouse or rabbit primary antibodies, this all-inclusive kit contains optimized reagents for blocking, antibody dilution, washing, HRP detection, and chemiluminescent development.

The included Pierce™ ECL Substrate generates a sensitive, long-lasting chemiluminescent signal, compatible with nitrocellulose and PVDF membranes. The protocol is easy to follow, requires no specialized instruments, and eliminates the risk of clogs or equipment maintenance common in vacuum-based systems.

Key Features & Benefits

• Ultra-Fast Protocol – Complete a Western blot in ~55 minutes, saving 4–5 hours compared to standard ECL workflows.
• High Sensitivity – Achieve results comparable to classic Pierce™ ECL methods.
• All-Inclusive Kit – Contains all reagents for blocking, antibody dilution, washing, and detection.
• No Special Equipment Needed – No vacuum pumps or disposable blotting trays required.
• Cost-Effective – Costs as little as $8 per blot; no extra consumables required.
• Excellent Stability – 1-year shelf life when stored at 4°C.
• Reproducible Results – Optimized for consistent performance with mouse and rabbit antibodies.

Applications

• Western Blotting – Rapid detection of proteins in research applications.
• Antibody Screening – Efficient testing of monoclonal and polyclonal antibodies.
• Protein Expression Analysis – Monitoring recombinant and native protein production.
• Reprobing Studies – Blots can be stripped and reprobed for multiple targets.

Kit Contents

Catalog No. 35050 – 1 L Kit (Sufficient for 25 Mini Blots)

• Fast Western Antibody Diluent – 500 mL
• Fast Western 10X Wash Buffer – 250 mL
• Fast Western Optimized HRP Reagent – 25 mL
• Pierce™ ECL Detection Reagent 1 – 125 mL
• Pierce™ ECL Detection Reagent 2 – 125 mL

Catalog No. 35055 – 200 mL Kit (Sufficient for 5 Mini Blots)

• Fast Western Antibody Diluent – 100 mL
• Fast Western 10X Wash Buffer – 50 mL
• Fast Western Optimized HRP Reagent – 5 mL
• Pierce™ ECL Detection Reagent 1 – 25 mL
• Pierce™ ECL Detection Reagent 2 – 25 mL

Technical Specifications

Ordering Information

The Thermo Scientific™ Pierce™ Fast Western Blot Kit, ECL Substrate offers a rapid, convenient, and cost-effective solution for high-quality Western blotting. With all reagents provided, compatibility with both mouse and rabbit primary antibodies, and no need for specialized equipment, this kit is an ideal choice for laboratories seeking fast turnaround and consistent, publication-ready results.