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Thermo Scientific™ Pierce™ Centrifuge Columns
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Specifications:
| Application | Protein Purification |
| Storage Temperature | Room Temperature |
| Product Type | columns |
| Product Brand | Thermo Fisher Scientific™ |
| Product Grade | Molecular Biology |
These centrifuge columns are constructed from low-protein-binding polypropylene and incorporate a polyethylene filter (~30-µm pore size) to retain resin beads while allowing efficient flow-through of samples and reagents. The twist-off bottoms simplify loading and flow control, and screw-cap closures help ensure sample containment.
Columns are compatible with spin or gravity-flow protocols, and their modular format enables flexible adaptation to a variety of protein purification and chromatography applications.
Available Formats and Capacities
| Catalog Number | Column Size | Max Volume | Recommended Resin Volume | Compatible Tube | Quantity |
|---|---|---|---|---|---|
| 89868 | 0.8 mL | 800 µL | 40–400 µL | Standard microcentrifuge | 50 |
| 89896 | 2 mL | 5 mL (2 mL resin + 3 mL sample) | 400 µL–2 mL | 15 mL conical tube | 25 |
| 89897 | 5 mL | 8 mL | 1–5 mL | 15 mL conical tube | 25 |
| 89898 | 10 mL | 22 mL | 2–10 mL | 50 mL conical tube | 25 |
| 89869 | 0.8 mL | 800 µL | 40–400 µL | Microcentrifuge | 200 |
Each column includes a twist-off bottom for flow control and a press-on cap for resealing.
Key Features
- Flexible format – Supports both centrifuge-based (spin) and gravity-flow chromatography.
- Reusable design – Ideal for multiple purification cycles when properly cleaned and maintained.
- Low protein binding – Ensures maximum recovery of target proteins.
- Broad compatibility – Suitable for agarose beads, Sepharose, ion exchange media, and gel filtration resins.
- Scalable volumes – From micro to preparative scale, with column capacities ranging from 0.8 mL to 20 mL.
- Lab-friendly fit – Compatible with standard 15 mL or 50 mL conical tubes.
Typical Applications
- Affinity chromatography
- Immuno-depletion or immunoprecipitation
- Ion exchange purification
- Gel filtration
- Buffer exchange and desalting
The Pierce™ Centrifuge Columns from Thermo Scientific are robust, reusable tools designed for efficient, small-scale protein purification in research laboratories. With multiple size options and compatibility with various workflows and sample volumes, they offer exceptional flexibility and performance for a wide range of applications in molecular biology, biochemistry, and proteomics.
- Capacity: 800 μL, 50 Columns 800 μL, 4 x 50 Columns 2 mL, 25 Columns 5 mL, 25 Columns 10 mL, 25 Columns