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Thermo Scientific™ PageRuler™ Prestained Protein Ladder, 10 to 180 kDa
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Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Protein Standards | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
Three-color, ready-to-use protein size standard for SDS-PAGE and western blotting
The Thermo Scientific™ PageRuler™ Prestained Protein Ladder contains a mixture of 10 pre-stained proteins (10–180 kDa) in blue, orange, and green for accurate protein sizing during SDS-polyacrylamide gel electrophoresis and western blotting.
Supplied in a ready-to-use format, this ladder requires no heating, reduction, or buffer addition before loading. It is compatible with both colorimetric and fluorescent detection methods, including NIR fluorescence (700 nm) and RGB fluorescence (555 nm), allowing for flexible imaging options.
The distinct colors make it easy to identify specific reference bands, enhancing migration monitoring during electrophoresis and protein transfer validation in western blotting.
Key Features & Benefits
- Wide Size Range – 10 markers spanning 10–180 kDa for precise protein sizing.
- Three-Color Visualization – Blue, orange, and green bands for easy reference during electrophoresis and blotting.
- Versatile Detection – Compatible with colorimetric, NIR (700 nm), and RGB (555 nm) fluorescence imaging.
- Ready-to-Use – No need for heating, reducing, or buffer addition before use.
- Gel System Compatibility – Works with multiple gel types, including Bolt™, Novex™, and NuPAGE™ formats.
Applications
- Protein Migration Monitoring – Track protein movement during SDS-PAGE runs.
- Protein Transfer Validation – Confirm transfer efficiency in western blotting.
- Protein Size Estimation – Accurate sizing of proteins in gels and blots.
Technical Specifications
| Parameter | Details |
|---|---|
| Product Type | Protein Ladder |
| Product Line | PageRuler™ |
| Molecular Weights | 10, 15, 25, 35, 40, 55, 70, 100, 130, 180 kDa |
| Number of Markers | 10 |
| Size Range | 10–180 kDa |
| Detection Methods | Colorimetric, NIR fluorescence (700 nm), RGB fluorescence (555 nm) |
| Stain Type | Blue, orange, green |
| Gel Compatibility | Bolt™ Bis-Tris Plus, Novex™ Tricine, Novex™ Tris-Glycine, NuPAGE™ Bis-Tris, NuPAGE™ Tris-Acetate, SDS-PAGE |
| Ready to Load | Yes |
| Unit Sizes | 26616 – 2 × 250 μL; 26616X4 – 8 × 250 μL; 26617 – 10 × 250 μL |
| Shipping Condition | Wet or dry ice |
| Storage Conditions | −20°C |
| Storage Buffer | 62.5 mM Tris-H₃PO₄ (pH 7.5), 1 mM EDTA, 2% SDS, 10 mM DTT, 1 mM NaN₃, 33% glycerol |
The Thermo Scientific™ PageRuler™ Prestained Protein Ladder is a highly versatile, three-color molecular weight marker that enhances accuracy and efficiency in SDS-PAGE and western blotting workflows. Its wide detection compatibility, distinctive color coding, and ready-to-use format make it an ideal standard for protein sizing and migration monitoring in both research and diagnostic development environments.
- Pack Size: 2 x 250 μL 8 x 250 μL 10 x 250 μL