Thermo Scientific™ Oxoid™ Mueller Hinton Agar (Dehydrated), 500G

Mueller Hinton Agar (MHA) is the gold standard medium for Antimicrobial Susceptibility Testing (AST), widely used in clinical and research settings. It is designed to meet both EUCAST and CLSI standards and is suitable for the Kirby-Bauer method and other internationally recognized AST procedures.

Key Features:

1. Standardization: Conforms to CLSI and EUCAST guidelines for AST, ensuring reliable and reproducible results.
2. Applications:
   • Disc diffusion testing
   • Minimum Inhibitory Concentration (MIC) testing
3. Starch Inclusion: Absorbs toxic factors produced during microbial growth, enabling growth from small inocula.
4. Clinical Utility: Recommended for testing a broad range of microorganisms, including:
   • Staphylococcus aureus (e.g., ATCC®25923)
   • Escherichia coli (e.g., ATCC®25922)
   • Pseudomonas aeruginosa (e.g., ATCC®27853)
   • Streptococcus pneumoniae
   • Haemophilus influenzae

Composition:

Preparation Instructions:

1. Suspend 38 g of medium in 1 liter of distilled water.
2. Heat to dissolve completely.
3. Autoclave at 121°C for 15 minutes.
4. Pour plates once cooled to 50°C.

Storage & Shelf Life:

• Dehydrated Medium: Store at 10–30°C, protected from moisture.
• Prepared Medium: Store at 2–8°C.

Applications:

1. Kirby-Bauer Disc Diffusion Method: The standard medium for this widely accepted AST method.
2. MIC Testing: For quantitative susceptibility assessment.
3. Microbial Testing Standards: Complies with ISO/TS 16782 for antimicrobial testing.

Key Microorganisms Tested:

References:

1. EUCAST Disc Diffusion Test Methodology.
2. CLSI Supplement M100S for AST Standards.
3. Bauer et al. (1966) Amer. J. Clin. Path.
4. Pollock et al. (1986) J. Clin. Microbiol.

Thermo Scientific™ Oxoid™ Mueller Hinton Agar is a high-quality, standardized medium for antimicrobial testing, supporting reliable determination of susceptibility profiles. Its compliance with major international standards (EUCAST and CLSI) makes it an indispensable tool in clinical microbiology and research.