Thermo Scientific™ o-Dianisidine, peroxidase substrate (3,3′-Dimethoxybenzidine, Fast Blue B) (copy)

1-Chloro-2,4-dinitrobenzene (CDNB) is a highly pure, yellow crystalline compound with a broad range of biochemical, analytical, and toxicological applications. With a molecular formula of C₆H₃ClN₂O₄ and a molecular weight of 202.55 g/mol, this compound is commonly used as a diagnostic reagent and research chemical, particularly in the enzymology and immunology sectors.

Chemical Properties:

• CAS Number: 97-00-7
• Molecular Formula: C₆H₃ClN₂O₄
• Molecular Weight: 202.55 g/mol
• Melting Point: 48–52 °C
• Boiling Point: 314–316 °C
• Density: 1.700 g/cm³
• Flash Point: 186 °C (366 °F)
• Storage Conditions: Ambient temperature
• UN Number: 3441
• ADR Classification: 6.1, II (toxic substances)

Applications:

• Enzyme Assays: CDNB is extensively used in glutathione S-transferase (GST) assays, where it acts as a chromogenic substrate to detect GST activity. It enables spectrophotometric measurement of enzyme kinetics in detoxification studies.
• Protein Research: It supports the quantification of GST subunits in human tissues via reverse-phase HPLC.
• Dermatological Models: Employed in immunotoxicology, it induces atopic dermatitis in BALB/c mouse models for allergy and inflammation research.
• Cell Signaling Studies: Acts as a contact sensitizer for evaluating cytokine release (e.g., IL-18) in human keratinocytes, facilitating skin immunology studies.

Preparation Note:

To prepare working solutions, CDNB is typically dissolved in ethanol and diluted in a phosphate buffer system (KH₂PO₄/Na₂HPO₄, pH 6.5) to a final concentration of 1 mM and 4% v/v ethanol.

Available Pack Sizes:

• 100 g (Cat. No. A13774.22)
• 500 g (Cat. No. A13774.36)
• 2.5 kg (Cat. No. A13774.0E)

Thermo Scientific™ 1-Chloro-2,4-dinitrobenzene is a highly specialized compound suitable for critical biochemical assays, immunological studies, and toxicology modeling. Its high purity (≥98%), stability, and defined reactivity make it a valuable reagent for research involving enzymatic detoxification, oxidative stress pathways, and immuno-inflammatory responses. Researchers in pharmacology, dermatology, and toxicology can rely on CDNB for precise and reproducible experimental outcomes.