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Thermo Scientific™ Invitrogen™ Qubit™ 1X dsDNA Broad Range (BR) Assay Kits
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Quantitation Range: 4 to 4000 ng
Specifications:
| Application | molecular biology, Fluorescence-Based Assay | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, Invitrogen | ||
| Product Grade | Molecular Biology | ||
The Invitrogen™ Qubit™ 1X dsDNA Broad Range (BR) Assay Kit provides a fast, highly accurate, and selective method for quantifying double-stranded DNA (dsDNA) across a broad concentration range. Designed for use with Qubit Fluorometers, this assay uses fluorescence-based detection to deliver superior accuracy compared to traditional UV absorbance methods, especially in the presence of contaminants.
The kit features a convenient 1X ready-to-use working solution, eliminating dilution steps and reducing preparation time. With excellent selectivity for dsDNA over RNA, single-stranded DNA, proteins, and free nucleotides, the assay ensures reliable quantification even in complex sample matrices.
This assay is ideal for laboratories performing next-generation sequencing (NGS), PCR, cloning, and other molecular biology workflows requiring precise DNA quantification.
Key Features
Broad Quantification Range
- Accurate quantification of dsDNA from 4 ng to 4,000 ng
- Suitable for medium to high concentration DNA samples
- Supports a wide range of molecular biology and genomics applications
Ready-to-Use 1X Working Solution
- Pre-formulated 1X assay reagent eliminates dilution steps
- Simplifies workflow and reduces preparation errors
- Improves reproducibility across experiments
High Selectivity for dsDNA
- Selectively quantifies double-stranded DNA
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Minimal interference from:
- RNA
- Single-stranded DNA
- Proteins
- Free nucleotides
- Salts, solvents, and detergents
Fast and Simple Workflow
- Requires only 1–20 μL of sample
- Results available within minutes
- Minimal hands-on time
Superior Accuracy Compared to UV Methods
- Fluorescence-based detection provides more reliable quantification
- Ideal for sensitive downstream applications such as NGS library preparation
Applications
The Qubit 1X dsDNA BR Assay Kit is ideal for a wide range of molecular biology and genomics applications, including:
- Next-generation sequencing (NGS) library preparation
- DNA quantification before Illumina sequencing workflows
- PCR and qPCR workflows
- Cloning and plasmid DNA quantification
- Genomic DNA quantification
- DNA sample normalization
- Research in genomics, biotechnology, and molecular biology
Instrument Compatibility
Designed for use with:
- Invitrogen™ Qubit™ 4 Fluorometer
- Invitrogen™ Qubit™ Flex Fluorometer
Kit Contents and Shipping
| Component | Shipping Condition | Storage |
|---|---|---|
| Qubit™ 1X dsDNA BR Working Solution | Ambient | Store as recommended |
| Qubit™ 1X dsDNA BR Lambda Standards | Wet Ice | Refrigerate upon arrival |
Technical Specifications
| Parameter | Specification |
|---|---|
| Assay Type | dsDNA Broad Range (BR) |
| Quantitation Range | 4–4,000 ng |
| Detection Method | Fluorescence |
| Sample Volume | 1–20 μL |
| Number of Assays | 100 or 500 |
| Instrument Compatibility | Qubit 4 and Qubit Flex Fluorometers |
| Selectivity | Highly selective for dsDNA |
| Format | Ready-to-use 1X solution |
| Unit Size | Each |
Advantages for Molecular Biology and NGS Laboratories
- Highly accurate DNA quantification for sequencing workflows
- Ready-to-use formulation simplifies workflow
- Superior selectivity over UV absorbance methods
- Ideal for Illumina NGS library preparation workflows such as Collibri kits
- Fast and reliable results for routine laboratory use
- Compatible with high-throughput genomics laboratories
The Invitrogen™ Qubit™ 1X dsDNA Broad Range (BR) Assay Kit is a reliable and efficient solution for precise quantification of double-stranded DNA across a wide concentration range. Its ready-to-use formulation, high selectivity, and compatibility with Qubit Fluorometers make it an essential tool for genomics laboratories, sequencing facilities, and molecular biology researchers requiring accurate DNA measurement for critical downstream applications such as NGS, PCR, and cloning workflows.
- Pack Size: 500 assays 100 assays