Thermo Scientific™ HEPES sodium salt 99%

Thermo Scientific's HEPES Sodium Salt is a high-purity biological buffer widely used in cell culture and biochemical applications. Its excellent buffering capacity, high solubility, and chemical stability make it an ideal choice for research requiring precise pH control.

Key Features

• Purity: ≥98.5% (by titration with HClO₄, on anhydrous substance)
• Form: White crystalline powder
• Buffering Range: pH 9.5–10.5 (1% solution in water)
• Chemical Stability: Minimal interference with biochemical reactions
• Water Content: ≤3% (by Karl Fischer titration)

Chemical and Physical Properties

Applications

1. Biological Buffer:
   • Maintains stable pH in cell culture media.
   • Effective for biochemical assays requiring a stable pH environment.
2. Biochemical and Enzymatic Research:
   • Ensures optimal enzymatic activity by stabilizing pH during reactions.
   • Suitable for protein studies and nucleic acid research.
3. Electrophoresis and Molecular Biology:
   • Used as a component in buffer systems for electrophoresis.

Advantages

• High Purity: Ensures reliability in sensitive biochemical applications.
• Low UV Absorbance: A(260 nm) ≤ 0.04 makes it suitable for optical applications.
• Chemical Stability: Resistant to degradation, ensuring long-term usability.
• Water Solubility: Dissolves readily, facilitating preparation of buffer solutions.

Specifications

Storage and Handling

• Storage Temperature: Store at ambient temperature in a dry environment.
• Shelf Life: Stable under recommended storage conditions.
• Packaging: Supplied as a white crystalline powder for ease of use.

Safety Information

• Hazard Classification: Irritant; handle with care.
• Precautions: Use appropriate protective equipment (gloves, goggles, and lab coat).
• Disposal: Follow local regulations for chemical waste disposal.

Thermo Scientific HEPES Sodium Salt 99% is a high-quality, reliable buffering agent suitable for a wide range of biochemical and molecular biology applications. Its high purity, stability, and low UV absorbance make it indispensable for research requiring precise pH control and optical transparency.