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Thermo Scientific™ GeneJET FFPE DNA Purification Kit
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Specifications:
| Application | Nucleic Acid Extraction | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Sample Prep | Forms | Kit with Various components |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ GeneJET FFPE DNA Purification Kit enables rapid and reliable isolation of high-quality genomic DNA from formalin-fixed, paraffin-embedded (FFPE) tissues. Designed for efficiency, the kit eliminates the need for toxic solvents such as xylene during deparaffinization, ensuring a safer workflow without compromising yield or quality.
Each preparation can process up to 8 sections (10 µm thick) of FFPE samples from a wide variety of tissue types, delivering up to 8 µg of genomic DNA in elution volumes as low as 20 µL. The DNA is highly pure and compatible with downstream molecular applications including PCR, qPCR, and next-generation sequencing (NGS) library preparation.
The complete protocol can be performed in less than 2 hours, making it an excellent choice for laboratories requiring fast and reproducible results.
Key Features & Benefits
- High-Quality DNA – Purified genomic DNA suitable for PCR, qPCR, and NGS workflows.
- Fast & Efficient – Complete procedure in under 2 hours; yields up to 8 µg DNA.
- Safe & Reliable – Eliminates the use of xylene and other toxic solvents.
- Flexible Elution – DNA can be eluted in volumes as low as 20 µL for concentrated samples.
- Proven Performance – High decrosslinking efficiency ensures robust amplification of DNA fragments (up to 1 kb).
Applications
- PCR amplification
- Real-time qPCR
- NGS library preparation
- DNA profiling from FFPE tissue samples
Kit Contents
- Proteinase K Solution
- RNase A Solution
- Digestion Buffer
- Binding Buffer
- Wash Buffer I (concentrated)
- Wash Buffer II (concentrated)
- Elution Buffer
- GeneJET DNA Purification Columns with Collection Tubes
- Extra Collection Tubes
- Detailed protocol
Product Specifications
| Parameter | Detail |
|---|---|
| Catalog Numbers | K0881 (50 preps), K0882 (250 preps) |
| Product Line | GeneJET |
| Product Type | FFPE DNA Purification Kit |
| Column Type | Spin column |
| Sample Type | FFPE, fixed samples |
| Target | Genomic DNA |
| Format | Spin column |
| Isolation Technology | Silica membrane spin column |
| DNA Yield | Up to 8 µg |
| Test Time | < 2 hours |
| Elution Volume | ≥20 µL |
| Compatibility | Not high-throughput compatible (manual use only) |
| For Use With (Applications) | PCR, qPCR, NGS library prep |
| Unit Size | Each |
| Regulatory Status | For Research Use Only. Not for use in diagnostic procedures. |
The Thermo Scientific™ GeneJET FFPE DNA Purification Kit delivers a fast, safe, and efficient method for extracting genomic DNA from FFPE tissue samples. With its ability to recover up to 8 µg of DNA in under 2 hours, free from toxic deparaffinization reagents, it provides an optimal solution for PCR, qPCR, and NGS applications where sample integrity and reproducibility are essential.
- Pack Size: For 50 preps For 50 Preps