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Specifications:
| Application | cell Signalling | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₂₂H₂₅NO₆ |
Colchicine is a potent alkaloid-derived secondary metabolite with well-documented antimitotic, anti-inflammatory, and cytotoxic properties. It exerts its primary biochemical effect by binding to tubulin, thereby inhibiting its polymerization and disrupting microtubule dynamics, making it a critical tool in cell cycle arrest, mitotic inhibition, and apoptosis studies.
This Thermo Scientific preparation of colchicine is supplied as a light yellow crystalline powder with ≥97% purity (determined by HPLC) and is suitable for biological, pharmacological, toxicological, and cellular research applications.
Available Pack Sizes
| Catalog Number | Pack Size | Container Type |
|---|---|---|
| 227125000 | 500 mg | Glass bottle for solids |
| 227120010 | 1 g | Glass bottle for solids |
| 227120050 | 5 g | Glass bottle |
| 227120250 | 25 g | Glass bottle |
Chemical & Physical Specifications
| Property | Specification |
|---|---|
| Purity (HPLC) | ≥96.0% (on anhydrous and solvent-free basis) |
| Appearance | Light yellow crystalline powder |
| Melting Point | 150–160 °C (decomposes) |
| Density | 1.32 g/cm³ (20 °C) |
| Water Content (K.F.) | ≤2% |
| Specific Optical Rotation | −240° to −250° (c = 1, alcohol, at 20 °C and 589 nm) |
| Infrared Spectrum | Conforms to reference standard |
Biochemical Actions & Mechanism
- Primary Action: Binds to tubulin, inhibiting its polymerization
- Disrupts: Microtubule assembly and dynamics
-
Cellular Effects:
- Inhibits mitosis
- Induces apoptosis
- Activates the JNK/SAPK pathway
- Suppresses cytokine secretion, fibroblast proliferation, and vesicle motility
-
Used to study:
- Leukocyte migration and diapedesis
- Lysosomal degranulation
- Collagen transport
Applications in Research
Colchicine has been used in:
- Genotoxicity assays: Sister-chromatid exchange (SCE) studies
- Antiviral/antibacterial research: Cytotoxic activity against pathogens
- Cytoskeletal studies: Microtubule depolymerization models
- Calcium signaling studies: Investigating Ca²⁺ transients in taxol-treated cells
- Anti-inflammatory and immunological research: Models of pericarditis, gout, familial Mediterranean fever, and Behçet’s disease
Storage and Handling
- Powder Stability: Stable at room temperature
- Light Sensitivity: Colchicine darkens upon light exposure; store protected from light
-
Solution Stability:
- Autoclavable and filter-sterilizable
- Stable ≥6 months in light-protected, sterilized solutions
- Stable in neutral and slightly alkaline solutions (pH ~8.1) when frozen
Thermo Scientific™ Colchicine (≥97%) is a high-purity, well-characterized small molecule essential for microtubule-targeted research, mitosis inhibition, and anti-inflammatory studies. With broad utility in cell biology, oncology, toxicology, and drug development, colchicine remains a foundational compound for investigating cytoskeletal function, inflammation, and cell cycle regulation. Its solid form is stable at room temperature and is packaged securely for laboratory use.
- Pack Size: 1g 5g 25g 500mg