Thermo Scientific™ Bisacrylamide 96%, Extra Pure

High-Purity Crosslinking Agent for Polyacrylamide Gel Electrophoresis (PAGE)

Thermo Scientific™ Bisacrylamide (N,N'-Methylenebisacrylamide) is a high-purity, crystalline crosslinker used to form polyacrylamide gels for protein and nucleic acid electrophoresis. When used with acrylamide, bisacrylamide enables the formation of stable gel matrices with tunable pore sizes, essential for accurate molecular separation in SDS-PAGE, native PAGE, and DNA/RNA gel electrophoresis.

This extra pure reagent, with ≥95% purity by HPLC and <1% water content, ensures reliable polymerization and reproducible gel performance, making it suitable for sensitive molecular biology and proteomics workflows.

Key Features & Benefits

• High Purity (≥96%) – Tested by HPLC to ensure consistent polymerization
• Crystalline Powder Form – Stable and easy to handle for precise weighing and solution preparation
• Low Moisture Content – <1% water content (Karl Fischer) to preserve reactivity and shelf life
• Reproducible Gel Casting – Supports high-resolution polyacrylamide gel formation
• Broad Electrophoresis Compatibility – Ideal for SDS-PAGE, native PAGE, and 2D gel systems

Applications

• Polyacrylamide Gel Electrophoresis (PAGE) – Protein and nucleic acid separation
• Crosslinking Agent in Gel Formation – Used with acrylamide to form controlled gel pore sizes
• 2D Electrophoresis and Western Blotting – For reproducible separation and transfer
• Molecular Biology & Biochemistry Research – Core reagent in analytical workflows

Chemical & Physical Specifications

Thermo Scientific™ Bisacrylamide 96%, Extra Pure is a reliable crosslinker for preparing polyacrylamide gels used in electrophoresis-based protein and nucleic acid analysis. Its high purity and consistent performance make it a trusted reagent for molecular biology, proteomics, and biochemical research workflows.