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Thermo Scientific™ Applied Biosystems™ BigDye™ Terminator v3.1 Cycle Sequencing Kit
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Specifications:
| Application | Capillary Electrophoresis | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Sequencing Buffer | Forms | Lyophilized DNA |
| Product Brand | Thermo Fisher Scientific™, Applied Biosystems™ | ||
| Product Grade | Molecular Biology | ||
The BigDye™ Terminator v3.1 Cycle Sequencing Kit provides a robust, high-fidelity solution for Sanger sequencing applications including de novo sequencing, resequencing, mutation detection, and finishing of complex templates. Its advanced dye chemistry ensures longer read lengths, improved base calling accuracy, and consistent peak heights, even in GC- or GT-rich and complex mixed-base regions.
Optimized for performance on a wide range of DNA template types and compatible with all Applied Biosystems capillary electrophoresis platforms, this kit is trusted by genomics researchers for producing reliable and reproducible sequence data at scale.
Key Features
- Superior signal balance with uniform peak heights
- Enhanced performance in AT-, GC-, and GT-rich templates
- Optimized for de novo and resequencing, mutation detection
- High compatibility with PCR products, plasmids, BACs, fosmids, and more
- Available in formats for both low- and high-throughput sequencing
- Compatible with 96- and 384-well thermal cycling formats
Specifications
| Property | Detail |
|---|---|
| Product Type | Sequencing Buffer (Fluorescent Dye Terminator) |
| Chemistry | BigDye™ Terminator v3.1 Ready Reaction Mix |
| Sequencing Technique | Sanger Sequencing – Dye Terminator Method |
| Applications | De Novo Sequencing, Resequencing, Mixed-base Detection, Germline Mutation |
| Template Compatibility | PCR Amplicons, Plasmid DNA, Fosmids, BAC DNA, Genomic & Lambda DNA |
| Read Length | Long-read, high signal fidelity |
| Compatible Equipment | SeqStudio™, 3500 Series, 3730 Series, GeneAmp™ 9700, Veriti™ Thermal Cyclers |
| Formats | 12-well strips, 8-well strips, 96/384-well plates |
| Storage Conditions | –15°C to –25°C; avoid repeated freeze-thaw cycles |
| Kit Contents | BigDye™ Ready Mix, M13 Primer, pREF-BDT™ Control DNA, 5X Sequencing Buffer |
| Unit Size | Each (various reaction sizes) |
| Regulatory Status | For Research Use Only; not for diagnostic use |
Packaging Options
| Catalog # | Reaction Size | Description |
|---|---|---|
| 4337454 | 24 Reactions | Small-scale Sanger sequencing kit |
| 4337455 | 100 Reactions | Mid-scale workflow applications |
| 4337456 | 1,000 Reactions | High-throughput labs |
| 4337457 | 5,000 Reactions | Extended high-volume processing |
| 4337458 | 25,000 Reactions | Large-scale sequencing centers and core facilities |
Applications
- DNA resequencing and variant detection
- De novo sequencing of unknown genomes
- Mixed-base heterozygote detection (50:50)
- Finishing reads for genomic assemblies
- Sequencing GC-rich or GT-rich amplicons
- Comparative sequencing and quality control
The BigDye™ Terminator v3.1 Cycle Sequencing Kit is the industry-standard reagent for accurate, reproducible Sanger sequencing. Designed for superior performance with complex templates and long read requirements, it enables high-quality data output across a wide range of research applications. Whether for small-volume projects or large-scale sequencing workflows, this kit delivers trusted results with streamlined thermal cycling and optimized chemistry.
- Reactions: 100 rxn 1000 rxn 5000 Rxns 24 Rxn 25000 Rxn