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Specifications:
| Application | cell Signalling | ||
| Storage Temperature | Room Temperature | ||
| Product Type | Laboratory Chemical | Forms | Powder |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Analytical grade | Formula | C₁₀H₁₃N₅O₄ |
Adenosine is a naturally occurring purine nucleoside composed of an adenine base linked to a ribose sugar. It plays a central role in energy metabolism, cell signaling, and neuromodulation. It is a fundamental biochemical compound present in RNA, and serves as the core structure in ATP, ADP, and AMP—key molecules involved in energy transfer and intracellular signaling pathways.
Thermo Scientific offers research-grade adenosine at ≥99% purity (HPLC), suitable for cell biology, molecular pharmacology, metabolism, and analytical chemistry applications.
Available Pack Sizes
| Catalog Number | Pack Size | Container |
|---|---|---|
| 164040050 | 5 g | Glass bottle |
| 164040250 | 25 g | Glass bottle |
| 164041000 | 100 g | Glass bottle |
| 164045000 | 500 g | Glass bottle |
Specifications
| Property | Specification |
|---|---|
| Purity (HPLC) | ≥99.0% |
| Appearance | White crystalline powder |
| Melting Point | 233–238 °C |
| Infrared Spectrum | Conforms to reference |
| Loss on Drying | ≤0.5% (105 °C, 2 hours) |
| Sulfated Ash | ≤0.1% |
| UV Absorbance (λmax 259 nm) | 98.0–102.0% (pH 7) |
| UV Transmittance (430 nm) | ≥98% (c = 0.5, H₂O, 1 cm cell) |
| A250/A260 Ratio | 0.76–0.80 |
| Nitrogen Content (N) | 25.69–26.73% (on dry basis) |
| Heavy Metals (as Pb) | ≤10 ppm |
Biochemical and Physiological Role
Adenosine functions as:
- A neuromodulator: Influences neurotransmitter release in the brain and spinal cord
- A signaling molecule: Binds to A₁, A₂A, A₂B, and A₃ adenosine receptors, which mediate physiological responses including vasodilation, immune suppression, and anti-inflammatory activity
- A cardioprotective agent: Plays a role in myocardial protection during ischemia
- An anti-inflammatory modulator: Suppresses proinflammatory cytokines (via A₂ receptors), but can be proinflammatory in pulmonary disorders like asthma and COPD
Research Applications
Adenosine has broad utility in:
-
Cell signaling & receptor studies
- Receptor-binding assays involving A₁–A₃ adenosine receptors
-
Stem cell and developmental biology
- Supplementation for osteogenic differentiation
-
Cancer biology
- Studies on glioblastoma stem-like cell (GSC) proliferation and survival
-
Metabolic and cardiovascular research
- Analysis of vasodilation and platelet aggregation mechanisms
-
Tissue dissociation protocols
- Component of collagenase solutions for adipose tissue digestion
Cited Applications
- Supplement in in vitro embryonic culture medium for osteogenic lineage differentiation
- Evaluation of cell viability and drug resistance using MTT and thymidine incorporation assays in cancer models
- Standard reference compound in high-performance liquid chromatography (HPLC) analyses
- Used in collagenase-mediated fat tissue digestion protocols in murine models
Handling and Storage
- Storage: Ambient conditions; keep in a dry, dark environment
- Solubility: Soluble in water; slightly soluble in alcohol
- Preparation: Prepare fresh aqueous solutions; aliquot and freeze for long-term storage
- Stability: Stable under recommended conditions for long-term use
Thermo Scientific™ Adenosine (≥99%) is a high-purity, research-grade nucleoside essential for studies in molecular signaling, metabolic regulation, neurotransmission, and immune modulation. Its pivotal role in cellular communication, cardiovascular physiology, and inflammation makes it a fundamental compound in biomedical research. This product is ideal for labs conducting work in receptor pharmacology, RNA synthesis, stem cell biology, and cancer therapeutics.
- Pack Size: 100g 500g 5g 25g