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Thermo Scientific™ Acrylamide 30% in aqueous solution
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Formula: C₃H₅NO
Specifications:
| Application | Protein Electrophoresis & Western Blotting | ||
| Storage Temperature | Ambient | ||
| Product Type | Molecular Biology Reagent | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™, thermo Scientific | ||
| Product Grade | Molecular Biology | Formula | C₃H₅NO |
High-Purity Monomer Solution for Polyacrylamide Gel Electrophoresis (PAGE) and Molecular Biology Applications
Thermo Scientific™ Acrylamide 30% (w/v) in Aqueous Solution is a ready-to-use electrophoresis-grade monomer solution designed for the preparation of polyacrylamide gels used in SDS-PAGE, native PAGE, and nucleic acid electrophoresis. Acrylamide polymerizes in the presence of a crosslinker (e.g., bis-acrylamide) and catalysts (e.g., APS/TEMED) to form a gel matrix ideal for size-based separation of proteins and nucleic acids.
This high-purity solution minimizes the formation of inhibitory contaminants, supporting reliable gel formation, reproducibility, and high-resolution band separation in research workflows.
Key Features & Benefits
- Electrophoresis-Grade Purity – Suitable for molecular biology, PAGE, and proteomics workflows
- 30% (w/v) Aqueous Solution – Pre-dissolved for easy dilution and custom gel preparation
- Water-Soluble – Homogeneous, ready-to-use format ensures consistent polymerization
- Versatile Use – Supports protein and nucleic acid separation, dye synthesis, and polymer production
- Research Use Only – Optimized for academic, proteomics, and biochemical applications
Applications
- Polyacrylamide Gel Electrophoresis (PAGE) – SDS-PAGE, native PAGE for protein analysis
- Nucleic Acid Electrophoresis – DNA/RNA separation in PAGE formats
- 2D Gel Electrophoresis – First-dimension IEF and second-dimension PAGE
- Chemical Synthesis – Intermediate for dyes, copolymers (e.g., contact lens materials), and polyacrylamide production
- Membrane Protein Extraction Studies – Referenced in high-impact proteomics workflows
Technical Specifications
| Attribute | Specification |
|---|---|
| Product Name | Acrylamide 30% in Aqueous Solution |
| Brand | Thermo Scientific™ |
| Concentration | 30% (w/v) |
| Form | Liquid (aqueous solution) |
| Solubility | Soluble in water |
| Application | Electrophoresis, PAGE, molecular biology |
| Use | Research Use Only (RUO) |
| Storage Conditions | Cool, dry, well-ventilated area; light-sensitive |
| Incompatibilities | Acids, oxidizing agents, iron/copper salts, free radical initiators |
| References | Molloy et al., Electrophoresis (1998); Rabilloud, Proteomics (2002) |
Thermo Scientific™ Acrylamide 30% Solution delivers consistent, high-performance results for researchers preparing polyacrylamide gels for electrophoresis. Pre-diluted and quality-controlled, it is ideal for use in protein separation, nucleic acid analysis, and a wide range of biochemical and proteomic applications.
- Pack Size: 1L 500 mL