Thermo Scientific™ Oxoid™ Bacillus Cereus Selective Supplement (Polymyxin B Supplement), 10 x 50,000 IU

The Thermo Scientific™ Oxoid™ Bacillus Cereus Selective Supplement (Polymyxin B Supplement) is a freeze-dried selective supplement specifically designed to inhibit the growth of Gram-negative bacteria, facilitating the isolation and enumeration of Bacillus cereus from food and fecal samples. Each vial contains 50,000 IU of Polymyxin B, suitable for supplementing 500mL of Bacillus Cereus Selective Agar Base (CM0617B) or MYP Agar Base (CM0929B).

This supplement is particularly useful for the preparation of PEMBA (Polymyxin Pyruvate Egg Yolk Mannitol Bromothymol Blue Agar), a widely recognized medium for Bacillus cereus detection in food safety and clinical microbiology laboratories.

Key Features

1. Selective Agent: Polymyxin B effectively inhibits Gram-negative bacteria, allowing specific recovery of Bacillus cereus.
2. Versatile Applications: Can be used with PEMBA medium and MYP Agar for the detection of Bacillus cereus in food and clinical samples.
3. High Potency: Each vial contains 50,000 IU of Polymyxin B for reliable performance in selective media.
4. Flexible Use: Each vial is designed to supplement 500mL of medium, providing cost-effective preparation.
5. Freeze-Dried Format: Ensures long shelf life and ease of reconstitution.

Specifications

Applications

1. Food Microbiology

• Detection and enumeration of Bacillus cereus in various food products, including dairy, meats, grains, and ready-to-eat foods.
• Preparation of PEMBA medium for food safety testing.

2. Clinical Microbiology

• Isolation of Bacillus cereus from fecal samples to investigate foodborne illnesses and infections.

3. Environmental and Industrial Microbiology

• Monitoring Bacillus cereus contamination in food processing environments and water samples.

Preparation Instructions

1. Reconstitution:
   • Aseptically reconstitute the contents of one vial (50,000 IU Polymyxin B) in a small volume of sterile distilled water.
2. Supplementing Media:
   • Add the reconstituted supplement to 500mL of Bacillus Cereus Selective Agar Base (CM0617B) or MYP Agar Base (CM0929B).
   • Mix thoroughly to ensure even distribution.
3. Pour Plates:
   • Pour the supplemented medium into sterile Petri dishes and allow it to solidify.

Advantages

1. Enhanced Selectivity: Effectively inhibits Gram-negative bacteria, improving the recovery and enumeration of Bacillus cereus.
2. Ease of Use: Freeze-dried format ensures simple reconstitution and consistent performance.
3. Versatility: Compatible with multiple media types, including PEMBA and MYP agar, for varied applications.
4. Cost-Effective: One vial prepares 500mL of medium, optimizing resource use.

Storage and Stability

• Storage Conditions: Store the freeze-dried supplement at 2–8°C in its original packaging.
• Shelf Life: Refer to the product label for expiration date.
• Post-Reconstitution: Use immediately or store reconstituted supplement at 2–8°C for short-term use.

Packaging

• Product: Bacillus Cereus Selective Supplement (Polymyxin B Supplement)
• Catalog Number: SR0099E
• Quantity: 10 vials (50,000 IU per vial)

The Thermo Scientific™ Oxoid™ Bacillus Cereus Selective Supplement is an indispensable tool for laboratories focusing on food safety, clinical diagnostics, and environmental microbiology. Its selective properties, high potency, and compatibility with PEMBA and MYP agar ensure reliable detection and enumeration of Bacillus cereus, supporting quality assurance and public health monitoring.