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Specifications:
| Application | Isothermal Amplification | ||
| Storage Temperature | -20°C | ||
| Product Type | PCR Reagent | Forms | Liquid |
| Product Brand | SolisBiodyne | ||
| Product Grade | Molecular Biology | ||
The SoliSD™ Bsm DNA Polymerase Kit is an exceptionally stable, high-performance isothermal amplification system based on the large fragment of DNA polymerase from Bacillus smithii. Enhanced with proprietary Stability TAG™ technology, this enzyme remains stable for at least 1 month at room temperature (37°C), enabling flexible handling, environmentally friendly shipping, and simplified reaction setup.
Engineered with strong strand displacement activity and lacking both 5’→3’ and 3’→5’ exonuclease activities, the enzyme is optimized for LAMP (loop-mediated isothermal amplification) and other isothermal applications. The kit’s unique SoliSD™ Supplement system improves reaction performance, eliminates non-template control (NTC) signals, and minimizes variability between replicates.
Key Features & Benefits
- Exceptional Stability – Remains stable for at least 1 month at RT (37°C).
- Fast Results – Amplification detectable in as little as 4 minutes.
- High Specificity – No background NTC signal.
- Strong Strand Displacement – Suitable for LAMP and other isothermal amplification methods.
- Flexible Performance – Works across 51–62°C (optimum at 60°C).
- Convenient Handling – Reactions can be set up at room temperature.
- Available Formats – Glycerol-free version also available for lyophilization workflows.
Kit Components (5-vial format)
- SoliSD™ Bsm DNA Polymerase (8 U/µL) – 1 vial
- 25× SoliSD™ Supplement – 1 vial
- 10× Isothermal Reaction Buffer – 1 vial
- 100 mM MgSO₄ – 1 vial
- 10× GC-rich Enhancer – 1 vial
Applications
- Loop-mediated isothermal amplification (LAMP)
- Rapid detection assays requiring fast turnaround
- Amplification of GC-rich templates
- Isothermal workflows with high reproducibility requirements
- Suitable for diagnostic research workflows (research use only)
Product Specifications
| Attribute | Detail |
|---|---|
| Polymerase Source | Large fragment DNA polymerase from Bacillus smithii |
| Activity | Strong strand displacement |
| Exonuclease Activity | None (no 5’→3’ or 3’→5’) |
| Optimum Temperature | 60°C (range 51–62°C) |
| Detection Time | From 4 minutes |
| Stability | ≥1 month at 37°C (RT) |
| Format | 5-vial kit with enzyme, buffers, enhancer, supplement |
| Storage | –20°C (routine) |
| Shipping | Room temperature |
Ordering Information
| Catalog Number | Size | Reaction Capacity |
|---|---|---|
| 32-21-0000S | 100 rxn | Small pack |
| 32-21-00250 | 250 rxn | Medium pack |
| 32-21-01000 | 1000 rxn | Large pack |
| 32-21-05000 | 5000 rxn | Bulk pack |
The SoliSD™ Bsm DNA Polymerase Kit offers an exceptionally stable, fast, and reliable enzyme system for isothermal DNA amplification. With Stability TAG™ technology, strong strand displacement activity, and unique supplement-enhanced performance, this kit is an ideal choice for LAMP assays, GC-rich template amplification, and high-throughput isothermal workflows requiring reproducibility and robustness.
- Pack Size: 250 reactions 1000 Reactions 100 rxn, free sample 5000 Reactions