Human Anti-LRRC25 Antibody (Anti-LRRC25) ELISA Kit

The Human Anti-LRRC25 Antibody (Anti-LRRC25) ELISA Kit is a sensitive and specific tool for the quantitative measurement of Anti-LRRC25 antibodies in human serum, plasma, culture media, or other biological fluids. This kit utilizes the Sandwich-ELISA method for precise and reliable results, making it ideal for research on immune responses, autoantibodies, and diseases related to LRRC25.

Purpose

• Quantification of Anti-LRRC25 Antibodies:
  • Detects and measures Anti-LRRC25 antibody levels in a variety of human biological samples.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids, including cell culture supernatants.

Principle

The assay employs a Sandwich-ELISA technique, providing a specific and quantitative measurement of Anti-LRRC25 antibodies. Below is the detailed workflow:

1. Pre-Coated Plate:
   • Microelisa strip plate wells are pre-coated with an LRRC25-specific antigen.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where Anti-LRRC25 antibodies in the sample bind to the pre-coated antigen.
3. HRP-Conjugated Detection:
   • An HRP-conjugated LRRC25 antigen is added, forming a sandwich complex with the captured Anti-LRRC25 antibodies.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. TMB Substrate Reaction:
   • A TMB substrate solution reacts with the HRP enzyme, producing a blue color in positive wells.
6. Stop Reaction and Measurement:
   • A stop solution is added, turning the color yellow. The optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Concentration Determination:
   • Anti-LRRC25 antibody concentrations are determined by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of Anti-LRRC25 antibodies with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of Anti-LRRC25 antibody levels using a standard curve.
4. User-Friendly Protocol:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Demonstrates consistent results across multiple experiments.

Kit Components

• Pre-coated Microelisa strip plate.
• Anti-LRRC25 standards for standard curve generation.
• HRP-conjugated LRRC25-specific antigen.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Autoimmune Disease Research:
  • Study the role of Anti-LRRC25 antibodies in autoimmune conditions.
• Immune Response Analysis:
  • Investigate antibody production in response to LRRC25.
• Biomarker Discovery:
  • Identify and quantify Anti-LRRC25 as a potential biomarker in clinical research.
• Drug Development:
  • Evaluate therapeutic interventions targeting LRRC25 or its associated pathways.
• Cell Biology and Immunology:
  • Explore LRRC25-related signaling pathways and immune responses.

Advantages

1. Accurate and Reliable:
   • High specificity for Anti-LRRC25 antibodies ensures precise results.
2. Convenient:
   • Ready-to-use components simplify preparation and execution.
3. Versatility:
   • Suitable for various sample types and experimental designs.
4. Reproducibility:
   • Consistent and reliable performance across multiple assays.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated LRRC25-specific antigen.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate Anti-LRRC25 antibody concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles.

The Human Anti-LRRC25 Antibody (Anti-LRRC25) ELISA Kit is a reliable and efficient method for detecting and quantifying Anti-LRRC25 antibodies in biological samples. Its high sensitivity, specificity, and straightforward protocol make it an invaluable tool for research into immune responses, autoimmune diseases, and LRRC25-related pathways.