Human Insulin like Growth Factor Binding Protein 7 (IGFBP-7) ELISA Kit

The Human Insulin-Like Growth Factor Binding Protein 7 (IGFBP-7) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of IGFBP-7 levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into cell growth, metabolism, cancer, and related disorders.

Purpose

• Quantification of IGFBP-7 Levels:
  • Detect and measure IGFBP-7 concentrations in human biological samples for research into growth factor binding, cell proliferation, and metabolic regulation.
• Sample Compatibility:
  • Suitable for serum, plasma, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique for the accurate and reliable quantification of IGFBP-7. The process involves:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to IGFBP-7.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where IGFBP-7 in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to IGFBP-7 is added, forming a sandwich complex with the captured IGFBP-7.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing IGFBP-7.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • IGFBP-7 concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of IGFBP-7 with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of IGFBP-7 concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a straightforward workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• IGFBP-7 standards for standard curve generation.
• HRP-conjugated IGFBP-7 detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Cancer Research:
  • Investigate IGFBP-7 as a biomarker for tumor growth and metastasis.
• Metabolic Studies:
  • Explore the role of IGFBP-7 in insulin resistance and glucose metabolism.
• Cell Proliferation and Apoptosis:
  • Study IGFBP-7’s role in cell growth, differentiation, and apoptosis.
• Cardiovascular Research:
  • Evaluate IGFBP-7 in vascular function and heart failure.
• Drug Development:
  • Monitor IGFBP-7 as a potential therapeutic target or biomarker for treatment efficacy.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of IGFBP-7.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated IGFBP-7 antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate IGFBP-7 concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human IGFBP-7 ELISA Kit is a valuable tool for researchers studying growth factor binding, cell metabolism, and related biological pathways. Its precision, user-friendly design, and broad compatibility make it indispensable for clinical and experimental applications.