Human Interleukin-15 receptor subunit Alpha (IL15RA) elisa Kit

The Human Interleukin-15 Receptor Subunit Alpha (IL15RA) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of IL15RA levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into immune regulation, cytokine signaling, and related disorders.

Purpose

• Quantification of IL15RA Levels:
  • Detect and measure IL15RA concentrations in human biological samples to study interleukin-15 receptor signaling and immune responses.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for accurate and reliable quantification of IL15RA. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to IL15RA.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where IL15RA in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to IL15RA is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing IL15RA.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • IL15RA concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of IL15RA with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of IL15RA concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Applications

• Immune Regulation Research:
  • Study IL15RA’s role in interleukin-15 signaling and immune system modulation.
• Cancer Research:
  • Investigate IL15RA involvement in tumor progression and immune evasion.
• Autoimmune Disorder Studies:
  • Assess IL15RA levels in conditions like rheumatoid arthritis and lupus.
• Infectious Disease Research:
  • Explore IL15RA in immune responses to bacterial and viral infections.
• Therapeutic Development:
  • Evaluate IL15RA as a target for immune-modulating therapies.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of IL15RA.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated IL15RA antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate IL15RA concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human IL15RA ELISA Kit is a critical tool for researchers studying immune signaling, cytokine pathways, and related disorders. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.