Human Carnitine Elisa Kit 96Tests

The Human Carnitine ELISA Kit is a precise and reliable tool designed for the quantitative detection of Carnitine levels in human serum, plasma, culture media, and other biological fluids. Using a Sandwich-ELISA technique, this kit enables accurate measurement of Carnitine, which is essential for research in energy metabolism, fatty acid oxidation, and related disorders.

Purpose

• Quantification of Carnitine Levels:
  • Measures Carnitine concentrations to assess its role in metabolism and health.
• Sample Compatibility:
  • Designed for use with human serum, plasma, and various biological fluids.

Principle

This assay employs a Sandwich-ELISA technique for the specific and quantitative measurement of Carnitine. Below is the workflow:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to Carnitine.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, allowing Carnitine in the sample to bind to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to Carnitine is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to reduce background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells with Carnitine.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • Carnitine concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Accurately detects low levels of Carnitine with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of Carnitine concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a straightforward workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• Carnitine standards for standard curve generation.
• HRP-conjugated Carnitine detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Metabolic Research:
  • Investigate Carnitine's role in energy metabolism and fatty acid oxidation.
• Nutritional Studies:
  • Assess Carnitine levels in relation to dietary intake and supplementation.
• Clinical Research:
  • Study Carnitine deficiencies and related metabolic disorders.
• Drug Development:
  • Evaluate the impact of therapies targeting Carnitine metabolism.
• Endocrinology and Metabolic Disorders:
  • Monitor Carnitine levels in conditions such as diabetes, obesity, and mitochondrial disorders.

Advantages

1. High Accuracy:
   • Ensures precise and reproducible quantification of Carnitine.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Provides consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated Carnitine antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate Carnitine concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Carnitine ELISA Kit provides a sensitive, accurate, and user-friendly solution for measuring Carnitine in biological samples. Its specificity and versatility make it an essential tool for metabolic research, clinical studies, and nutritional assessments.