Human Serum Ferritin, SF ELISA Kit

The Human Serum Ferritin (SF) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of ferritin levels in human serum, plasma, culture media, and other biological fluids. Ferritin is a critical biomarker for iron storage and metabolism, and this kit is essential for research in iron-related disorders and metabolic studies.

Purpose

• Quantification of SF Levels:
  • Detect and measure ferritin concentrations in human biological samples to study iron metabolism and related health conditions.
• Sample Compatibility:
  • Suitable for human serum, plasma, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for the accurate and reliable quantification of serum ferritin. Below is the process:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to SF.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where ferritin in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to SF is added, forming a sandwich complex with the captured ferritin.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing SF.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • Ferritin concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of SF with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Suitable for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of SF concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• Ferritin standards for standard curve generation.
• HRP-conjugated SF detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Iron Metabolism Research:
  • Study ferritin as a biomarker for iron storage and metabolism.
• Clinical Diagnostics Research:
  • Assess SF levels in conditions like iron deficiency anemia, hemochromatosis, and chronic diseases.
• Inflammation Studies:
  • Investigate ferritin as an acute-phase reactant in inflammatory conditions.
• Metabolic Research:
  • Explore SF levels in metabolic syndrome and chronic diseases.
• Therapeutic Development:
  • Monitor ferritin as a biomarker in drug development targeting iron metabolism.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of SF.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated SF antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate SF concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Serum Ferritin (SF) ELISA Kit is an essential tool for researchers studying iron metabolism, chronic diseases, and inflammatory conditions. Its precision, user-friendly design, and broad compatibility make it indispensable for clinical and experimental applications.