Human Total Cholesterol Elisa Kit – 96T

The Human Total Cholesterol (TC) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of total cholesterol (TC) levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into lipid metabolism, cardiovascular health, and related disorders.

Purpose

• Quantification of TC Levels:
  • Detect and measure total cholesterol concentrations in human biological samples to study lipid metabolism and cardiovascular health.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique to ensure accurate and reliable quantification of TC. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to TC.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where TC in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to TC is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing TC.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • TC concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of TC with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of TC concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• TC standards for standard curve generation.
• HRP-conjugated TC detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Lipid Metabolism Research:
  • Study TC’s role in cholesterol biosynthesis and transport.
• Cardiovascular Health Studies:
  • Investigate TC levels as a biomarker for atherosclerosis, heart disease, and metabolic syndrome.
• Clinical Diagnostics Research:
  • Assess TC as a biomarker for hypercholesterolemia and lipid-related disorders.
• Nutritional and Metabolic Studies:
  • Explore the relationship between dietary cholesterol and serum TC levels.
• Therapeutic Development:
  • Evaluate treatments targeting cholesterol metabolism and cardiovascular health.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of TC.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated TC antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate TC concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Total Cholesterol (TC) ELISA Kit is a vital tool for researchers studying lipid metabolism, cardiovascular health, and related metabolic disorders. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.