Human folate ELISA Kit

The Human Folate ELISA Kit is a sensitive and specific assay designed for the quantitative detection of reduced folate levels in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research into vitamin metabolism, nutritional health, and related disorders.

Purpose

• Quantification of Reduced Folate Levels:
  • Detect and measure reduced folate concentrations in human biological samples to study vitamin B9 metabolism and associated health conditions.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique to ensure accurate and reliable quantification of reduced folate. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to reduced folate.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where reduced folate in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to reduced folate is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing reduced folate.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • Reduced folate concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of reduced folate with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of reduced folate concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• Reduced folate standards for standard curve generation.
• HRP-conjugated reduced folate detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Nutritional Research:
  • Study folate levels in vitamin B9 metabolism and dietary health.
• Pregnancy and Fetal Health Studies:
  • Investigate reduced folate levels in maternal health and neural tube defect prevention.
• Metabolic Research:
  • Explore folate’s role in amino acid metabolism and DNA synthesis.
• Clinical Diagnostics Research:
  • Monitor folate levels in diseases associated with folate deficiency or excess, including anemia and neurological disorders.
• Therapeutic Development:
  • Evaluate treatments targeting folate metabolism and vitamin supplementation.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of reduced folate.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated reduced folate antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate reduced folate concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Folate ELISA Kit is a vital tool for researchers studying vitamin metabolism, nutritional health, and related disorders. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.