Human growth differentiation factor 15 (GDF15) ELISA Kit

The Human Growth Differentiation Factor 15 (GDF15) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of GDF15 levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into metabolic regulation, stress responses, and disease biomarkers.

Purpose

• Quantification of GDF15 Levels:
  • Detect and measure GDF15 concentrations in human biological samples to study stress signaling, metabolic regulation, and disease pathways.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for the accurate and reliable quantification of GDF15. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to GDF15.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where GDF15 in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to GDF15 is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing GDF15.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • GDF15 concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of GDF15 with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of GDF15 concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• GDF15 standards for standard curve generation.
• HRP-conjugated GDF15 detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Metabolic Regulation Research:
  • Study GDF15’s role in energy homeostasis and metabolic disorders.
• Stress and Cellular Damage Studies:
  • Investigate GDF15 as a biomarker for cellular stress and mitochondrial dysfunction.
• Cancer Research:
  • Assess GDF15 levels in tumor microenvironments and cancer progression.
• Cardiovascular and Inflammatory Research:
  • Monitor GDF15 in cardiovascular diseases and inflammatory conditions.
• Therapeutic Development:
  • Evaluate treatments targeting GDF15 pathways for metabolic and cancer therapies.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of GDF15.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated GDF15 antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate GDF15 concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Growth Differentiation Factor 15 (GDF15) ELISA Kit is a critical tool for researchers studying metabolic regulation, stress responses, and disease biomarkers. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.