Human Transforming Growth factor beta1,TGF-beta1 ELISA kit

The Human Transforming Growth Factor Beta 1 (TGFβ1) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of TGFβ1 levels in human serum, plasma, culture media, and other biological fluids. This kit is essential for research into cell signaling, immune regulation, fibrosis, and cancer biology.

Purpose

• Quantification of TGFβ1 Levels:
  • Detect and measure TGFβ1 concentrations in human biological samples to study immune regulation, fibrosis, and cell growth signaling pathways.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique for the accurate and reliable quantification of TGFβ1. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to TGFβ1.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where TGFβ1 in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to TGFβ1 is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing TGFβ1.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • TGFβ1 concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of TGFβ1 with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of TGFβ1 concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• TGFβ1 standards for standard curve generation.
• HRP-conjugated TGFβ1 detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Cell Signaling Research:
  • Study TGFβ1’s role in growth, differentiation, and apoptosis.
• Immune Regulation Studies:
  • Investigate TGFβ1 in immune modulation and inflammatory responses.
• Fibrosis Research:
  • Explore TGFβ1 as a biomarker for fibrosis in organs such as the liver, kidney, and lungs.
• Cancer Research:
  • Assess TGFβ1 levels in tumor microenvironments and its role in cancer progression and metastasis.
• Therapeutic Development:
  • Monitor TGFβ1 as a potential therapeutic target in fibrosis and cancer treatment.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of TGFβ1.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated TGFβ1 antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate TGFβ1 concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Transforming Growth Factor Beta 1 (TGFβ1) ELISA Kit is a critical tool for researchers studying cell signaling, immune regulation, fibrosis, and cancer biology. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.