Human Thyroxine-Binding Globulin Assay, TBG ELISA Kit

The Human Thyroxine-Binding Globulin (TBG) ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of TBG levels in human serum, plasma, urine, culture media, and other biological fluids. This kit is essential for research into thyroid hormone transport, endocrine health, and related metabolic disorders.

Purpose

• Quantification of TBG Levels:
  • Detect and measure thyroxine-binding globulin concentrations in human biological samples for research into thyroid hormone transport and metabolism.
• Sample Compatibility:
  • Suitable for serum, plasma, urine, culture media, and other biological fluids.

Principle

The assay employs a Sandwich-ELISA technique to ensure accurate and reliable quantification of TBG. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to TBG.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where TBG in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to TBG is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing TBG.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • TBG concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of TBG with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, urine, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of TBG concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• TBG standards for standard curve generation.
• HRP-conjugated TBG detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Thyroid Hormone Transport Research:
  • Study TBG’s role in binding and transporting thyroid hormones like T3 and T4.
• Endocrine Studies:
  • Investigate TBG levels in hyperthyroidism, hypothyroidism, and other thyroid dysfunctions.
• Clinical Diagnostics Research:
  • Assess TBG as a biomarker for thyroid health and hormone regulation.
• Reproductive and Prenatal Health:
  • Explore the relationship between TBG and thyroid hormone availability during pregnancy.
• Therapeutic Development:
  • Evaluate treatments targeting thyroid hormone transport and regulation.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of TBG.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated TBG antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate TBG concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Thyroxine-Binding Globulin (TBG) ELISA Kit is a vital tool for researchers studying thyroid hormone transport, endocrine health, and metabolic disorders. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.