Human Testosterone Elisa Kit, 96 Tests In 96 Well Plate

The Human Testosterone ELISA Kit is a sensitive and specific assay designed for the quantitative detection of testosterone levels in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research into reproductive health, endocrine function, and hormone-related conditions.

Purpose

• Quantification of Testosterone Levels:
  • Detect and measure testosterone concentrations in human biological samples to study hormone regulation, reproductive health, and metabolic processes.
• Sample Compatibility:
  • Suitable for serum, plasma, culture media, and other biological fluids.

Principle

The assay utilizes a Sandwich-ELISA technique for the accurate and reliable quantification of testosterone. The process includes:

1. Pre-Coated Plate:
   • Microelisa stripplate wells are pre-coated with an antibody specific to Testosterone.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where testosterone in the sample binds to the immobilized antibody.
3. HRP-Conjugated Detection:
   • A Horseradish Peroxidase (HRP)-conjugated antibody specific to testosterone is added, forming a sandwich complex.
4. Washing Step:
   • Free and unbound components are washed away to minimize background interference.
5. Substrate Reaction:
   • TMB substrate solution reacts with HRP, producing a blue color in wells containing testosterone.
6. Stop Reaction and Measurement:
   • A stop solution is added, changing the color from blue to yellow. Optical density (OD) is measured at 450 nm using a spectrophotometer.
7. Quantification:
   • Testosterone concentrations are calculated by comparing OD values to a standard curve.

Key Features

1. High Sensitivity and Specificity:
   • Detects low levels of testosterone with minimal cross-reactivity.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, culture media, and other biological fluids.
3. Quantitative Results:
   • Provides precise measurement of testosterone concentrations using a standard curve.
4. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents for a streamlined workflow.
5. Reproducibility:
   • Ensures consistent and reliable results across multiple experiments.

Kit Components

• Pre-coated Microelisa stripplate.
• Testosterone standards for standard curve generation.
• HRP-conjugated testosterone detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Applications

• Reproductive Health Research:
  • Study testosterone’s role in fertility, sexual health, and puberty.
• Endocrine Studies:
  • Investigate testosterone levels in hormonal balance and its interactions with other sex hormones.
• Clinical Diagnostics Research:
  • Assess testosterone as a biomarker for hypogonadism, infertility, and androgen disorders.
• Sports and Exercise Physiology:
  • Monitor testosterone levels in response to training, supplementation, or anabolic treatments.
• Therapeutic Development:
  • Evaluate testosterone-related treatments and hormone replacement therapies.

Advantages

1. High Accuracy:
   • Provides precise and reproducible quantification of testosterone.
2. Convenience:
   • Ready-to-use reagents simplify preparation and assay execution.
3. Versatility:
   • Compatible with various biological sample types.
4. Reliable:
   • Delivers consistent results for robust data interpretation.

Assay Protocol Summary

1. Add standards or samples to the pre-coated wells.
2. Incubate with HRP-conjugated testosterone antibody.
3. Wash to remove unbound components.
4. Add TMB substrate to develop color.
5. Stop the reaction and measure OD at 450 nm.
6. Calculate testosterone concentrations using the standard curve.

Storage and Stability

• Store all kit components at 2–8°C to maintain reagent integrity.
• Avoid repeated freeze-thaw cycles to ensure assay reliability.

The Human Testosterone ELISA Kit is a critical tool for researchers investigating testosterone’s role in hormonal regulation, reproductive health, and endocrine function. Its precision, ease of use, and broad compatibility make it indispensable for clinical and experimental applications.