Human Estradiol - E2 Elisa Kit, 96 Tests In 96 Well Plate

Human Estradiol ELISA Kit

The Human Estradiol ELISA Kit is a highly sensitive and specific assay designed for the quantitative detection of Estradiol (E2) levels in human serum, plasma, urine, culture media, and other biological fluids. This kit is ideal for research in reproductive health, hormonal regulation, and related conditions.

Sample Preparation

1. Serum Preparation

1. Collect whole blood and allow it to clot at room temperature for 10–20 minutes.
2. Centrifuge at 2,000–3,000 rpm for 20 minutes to separate the serum.
3. Re-centrifuge if precipitates appear during storage.

2. Plasma Preparation

1. Collect whole blood into tubes containing anticoagulants (e.g., EDTA or citrate).
2. Incubate at room temperature for 10–20 minutes.
3. Centrifuge at 2,000–3,000 rpm for 20 minutes to obtain plasma.
4. Re-centrifuge if necessary.

3. Urine Samples

1. Collect urine in aseptic tubes.
2. Centrifuge at 2,000–3,000 rpm for 20 minutes and carefully collect the supernatant.
3. Re-centrifuge if precipitates form during storage.

4. Cell Samples

1. For cell secretions, collect culture supernatants in aseptic tubes and centrifuge at 2,000–3,000 rpm for 20 minutes.
2. For intracellular components:
   • Dilute cells in PBS (pH 7.2–7.4) at 1×10⁷/mL.
   • Disrupt cells via repeated freeze-thaw cycles.
   • Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

5. Tissue Samples

1. Cut and weigh tissue samples, then freeze in liquid nitrogen and store at -80°C.
2. Homogenize samples in PBS (pH 7.4) at 4°C.
3. Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

Important Notes

1. Perform ELISA assays as soon as possible after sample collection.
2. Store samples at -20°C if immediate testing is not feasible.
3. Avoid repeated freeze-thaw cycles to preserve sample integrity.
4. Do not use samples containing sodium azide (NaN₃) as it inhibits HRP activity.

Applications

• Reproductive Health Research:
  • Study estradiol levels in relation to ovulation, menstruation, and fertility.
• Hormonal Regulation Studies:
  • Investigate estradiol’s role in endocrine function and hormonal balance.
• Clinical Diagnostics Research:
  • Assess estradiol as a biomarker in hormonal disorders, menopause, and infertility.
• Cancer Research:
  • Study estradiol levels in hormone-sensitive cancers such as breast and ovarian cancer.
• Drug Development:
  • Evaluate therapeutic interventions targeting estrogen pathways.

Advantages

1. High Sensitivity and Specificity:
   • Detects low levels of estradiol with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, urine, cell culture supernatants, and tissue homogenates.
3. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents.
4. Reproducibility:
   • Provides consistent and reliable results across multiple experiments.

Storage and Stability

• Store all kit components at 2–8°C.
• Avoid repeated freeze-thaw cycles to maintain reagent integrity.

The Human Estradiol ELISA Kit is an essential tool for researchers investigating estradiol's role in reproductive health, hormonal balance, and related diseases. Its precision, user-friendly design, and broad compatibility make it invaluable for various clinical and experimental applications.