Human Cytochrome C, Cyt-C Elisa Kit, 96-Tests Per Kit

Human Cytochrome C (Cyt-C) ELISA Kit

The Human Cytochrome C (Cyt-C) ELISA Kit is a sensitive and specific assay designed for the quantitative detection of Cytochrome C levels in human serum, plasma, culture media, and other biological fluids. This kit is ideal for research on apoptosis, mitochondrial function, and oxidative stress-related disorders.

Sample Preparation

1. Serum Preparation

1. Collect whole blood and allow it to clot at room temperature for 10–20 minutes.
2. Centrifuge at 2,000–3,000 rpm for 20 minutes to separate serum.
3. Re-centrifuge if any precipitates form during storage.

2. Plasma Preparation

1. Collect whole blood into tubes containing anticoagulants (e.g., EDTA or citrate).
2. Incubate at room temperature for 10–20 minutes.
3. Centrifuge at 2,000–3,000 rpm for 20 minutes to obtain plasma.
4. Re-centrifuge if necessary.

3. Urine Samples

1. Collect urine in aseptic tubes.
2. Centrifuge at 2,000–3,000 rpm for 20 minutes and carefully collect the supernatant.
3. Re-centrifuge if precipitates form during storage.

4. Cell Samples

1. For cell secretions, collect culture supernatants in aseptic tubes and centrifuge at 2,000–3,000 rpm for 20 minutes.
2. For intracellular components:
   • Dilute cells in PBS (pH 7.2–7.4) at 1×10⁷/mL.
   • Disrupt cells via repeated freeze-thaw cycles.
   • Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

5. Tissue Samples

1. Cut and weigh tissue samples, then freeze in liquid nitrogen and store at -80°C.
2. Homogenize samples in PBS (pH 7.4) at 4°C.
3. Centrifuge at 2,000–3,000 rpm for 20 minutes and collect the supernatant.

Important Notes

1. Perform ELISA assays as soon as possible after sample collection.
2. Store samples at -20°C if immediate testing is not feasible.
3. Avoid repeated freeze-thaw cycles to preserve sample integrity.
4. Do not use samples containing sodium azide (NaN₃) as it inhibits HRP activity.

Applications

• Apoptosis Research:
  • Quantify Cyt-C release as a marker for programmed cell death.
• Mitochondrial Function Studies:
  • Investigate Cyt-C in mitochondrial integrity and function.
• Oxidative Stress Research:
  • Study Cyt-C as a biomarker for oxidative damage.
• Neurological Disorders:
  • Assess Cyt-C levels in conditions such as Parkinson’s and Alzheimer’s diseases.
• Cancer Research:
  • Evaluate Cyt-C as a marker for cancer cell apoptosis and treatment response.
• Drug Development:
  • Analyze the impact of therapeutic agents on Cyt-C-mediated pathways.

Advantages

1. High Sensitivity and Specificity:
   • Detects low levels of Cyt-C with minimal cross-reactivity or interference.
2. Wide Sample Compatibility:
   • Validated for serum, plasma, urine, cell culture supernatants, and tissue homogenates.
3. Ease of Use:
   • Includes pre-coated plates and ready-to-use reagents.
4. Reproducibility:
   • Provides consistent and reliable results across multiple experiments.

Storage and Stability

• Store all kit components at 2–8°C.
• Avoid repeated freeze-thaw cycles to maintain reagent integrity.

The Human Cytochrome C (Cyt-C) ELISA Kit is an essential tool for researchers investigating apoptosis, mitochondrial health, and oxidative stress. Its precision, user-friendly design, and broad compatibility make it invaluable for various clinical and experimental applications.