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Human Anti-Core Antigen Of Hepatitis B Virus Antibody, Anti-Hbcab Elisa Kit, 96 Tests
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Specifications:
| Application | ELISA-Based Assays |
| Storage Temperature | 2-8°C |
| Product Type | Elisa Kit |
| Product Brand | SUNLONG BIOTECH |
| Product Grade | Molecular Biology |
The Human HBcAb ELISA Kit (Qualitative) is a robust and reliable diagnostic tool designed for the qualitative detection of anti-hepatitis B virus core antibodies (HBcAb) in human biological samples such as serum, plasma, urine, cell culture supernatants, and tissue samples. This kit utilizes a sandwich ELISA method to ensure high specificity and sensitivity, making it ideal for research, clinical, and epidemiological applications.
Purpose
- Detect and identify anti-HBV core antibodies in human samples to assess past or ongoing infection with hepatitis B virus (HBV).
- Support research into HBV infection and immunity.
- Provide a qualitative evaluation of HBV serostatus for epidemiological studies.
Principle of the Assay
The assay employs the sandwich ELISA technique:
- Antigen Capture:
- The microplate is pre-coated with HBcAg, specific for anti-HBc antibodies.
- Sample Binding:
- When biological samples containing HBcAb are added to the wells, the antibody binds to the antigen on the plate.
- Detection:
- An enzyme-labeled secondary antibody binds to the HBcAb-antigen complex.
- Signal Development:
- TMB substrate is added, which reacts with the enzyme to produce a colorimetric signal.
- The signal intensity correlates with the presence of HBcAb in the sample.
- Qualitative Results:
- The assay provides a positive or negative result based on a pre-determined cutoff value.
Kit Components
The kit contains all necessary reagents and materials for performing the ELISA:
| Component | Description |
|---|---|
| Pre-Coated Microelisa Stripplate | HBcAg-coated wells for antigen binding. |
| Standard Diluent | For preparing sample dilutions. |
| HRP-Conjugated Detection Antibody | Specific for HBcAb, conjugated with HRP enzyme. |
| Wash Buffer | Removes unbound substances during washing. |
| TMB Substrate Solution | Develops a colorimetric signal for detection. |
| Stop Solution | Stops the enzymatic reaction and stabilizes the color. |
| Positive and Negative Controls | To validate assay performance and cutoff determination. |
| Plate Sealer | Prevents evaporation during incubation. |
| Instruction Manual | Detailed protocol for accurate assay execution. |
Applications and Use Cases
1. Clinical Research:
- HBV Infection Assessment:
- Detect HBcAb to determine if a patient has been previously exposed to HBV or has a chronic infection.
- Serological Testing:
- Used in conjunction with other HBV markers (e.g., HBsAg, anti-HBs) to complete HBV serostatus evaluations.
2. Public Health and Epidemiology:
- HBV Surveillance:
- Monitor HBV prevalence in populations to assess vaccination program effectiveness.
- Outbreak Investigations:
- Identify HBV exposure sources and track transmission patterns.
3. Vaccine and Immunology Studies:
- Vaccine Efficacy Monitoring:
- Assess immune response following vaccination against HBV.
- Antibody Response Analysis:
- Study host immune responses to HBV core antigens in different clinical conditions.
4. Drug and Therapeutic Research:
- Antiviral Therapy Development:
- Screen patient samples for HBcAb levels during clinical trials for HBV treatments.
- Monitoring HBV Reactivation:
- Detect potential reactivation of latent HBV in patients undergoing immunosuppressive therapy.
5. Laboratory Diagnostics:
- Serological Screening:
- Aids in diagnosing acute or chronic HBV infections in diagnostic workflows.
- Donor Screening:
- Screen blood and organ donors to prevent HBV transmission.
Features and Benefits
- High Sensitivity and Specificity:
- Accurately detects HBcAb with minimal cross-reactivity.
- User-Friendly Design:
- Ready-to-use reagents and a pre-coated microplate streamline the workflow.
- Comprehensive Kit:
- Includes controls for validation, ensuring accurate and reliable results.
- Wide Sample Compatibility:
- Compatible with serum, plasma, urine, cell culture supernatants, and tissue samples.
- Fast and Reliable Results:
- Provides qualitative outcomes within a few hours, enabling timely decision-making.
Sample Preparation Overview
1. Serum:
- Collect whole blood, allow clotting, and centrifuge at 2,000–3,000 rpm for 20 minutes.
2. Plasma:
- Collect anticoagulated blood (EDTA or citrate) and centrifuge at 2,000–3,000 rpm for 20 minutes.
3. Urine:
- Centrifuge at 2,000–3,000 rpm for 20 minutes, and collect the clear supernatant.
4. Cell Culture Supernatants:
- Centrifuge cultured media at 2,000–3,000 rpm for 20 minutes, and collect the supernatant.
5. Tissue Samples:
- Homogenize tissue in PBS (pH 7.4), centrifuge at 2,000–3,000 rpm for 20 minutes, and collect the supernatant.
Assay Workflow
- Add standards, controls, and prepared samples to the pre-coated plate.
- Incubate and wash to remove unbound components.
- Add HRP-conjugated antibody and incubate.
- Wash and add TMB substrate to develop the signal.
- Stop the reaction and measure OD at 450 nm.
- Interpret results as positive or negative based on the provided cutoff values.
Storage Conditions
- Store all reagents at 2–8°C.
- Avoid repeated freeze-thaw cycles to preserve reagent integrity.
Advantages of the Human HBcAb ELISA Kit
- Accuracy: Reliable qualitative detection of HBcAb.
- Versatility: Compatible with multiple biological sample types.
- Efficiency: Rapid results with easy-to-follow protocol.
- Cost-Effective: Comprehensive kit minimizes additional reagent purchases.
The Human HBcAb ELISA Kit (Qualitative) is an essential tool for the qualitative detection of anti-HBc antibodies, providing critical information for HBV research, public health, and clinical diagnostics. Its high sensitivity, specificity, and ease of use make it indispensable for laboratories seeking reliable serological screening of HBV infections.