Sigma-Aldrich Chloramphenicol, BioReagent, suitable for plant cell culture

Broad-Spectrum Antibiotic for Microbiology, Molecular Biology, and Plant Tissue Culture

Sigma-Aldrich Chloramphenicol (BioReagent, Cell Culture Grade) is a high-purity, broad-spectrum antibiotic widely used in plant cell culture, molecular biology, and microbiology research. It is a synthetic antibiotic originally derived from Streptomyces venezuelae and is effective against Gram-positive, Gram-negative bacteria, mycoplasma, and certain mycobacteria.

With its high stability, endotoxin-tested purity, and ability to inhibit bacterial protein synthesis, Chloramphenicol is widely used in molecular biology applications for bacterial selection (10–20 µg/mL) and as a selection agent for transformed plant cells containing chloramphenicol resistance genes.

Key Features & Benefits

✅ High-Purity BioReagent Grade – Ensures consistent and reproducible results in cell culture and microbiology applications

✅ Broad-Spectrum Antibiotic – Effective against Gram-positive & Gram-negative bacteria, mycoplasma, and mycobacteria

✅ Validated for Plant Cell Culture & Bacterial Selection – Suitable for genetic transformation and antibiotic resistance studies

✅ Mode of Action: Protein Synthesis Inhibitor – Blocks peptidyl transferase activity in the 50S ribosomal subunit

✅ Endotoxin-Tested & Highly Stable – Maintains potency over a wide pH range and in aqueous solutions

✅ Long-Term Storage Stability – Retains activity at 2–8°C, with aqueous solutions stable for months

✅ Compatible with Molecular Biology & Genetic Engineering – Used in plasmid selection, recombinant protein expression, and gene cloning

Technical Specifications

Mechanism of Action & Resistance

Mode of Action:

• Chloramphenicol inhibits bacterial protein synthesis by binding to the 50S ribosomal subunit, blocking peptidyl transferase activity.
• This prevents amino acid chain elongation, leading to cell growth inhibition and bacterial death.
• It also inhibits mitochondrial and chloroplast protein synthesis, making it effective for plant cell culture studies.

Mode of Resistance:

• Bacteria expressing chloramphenicol acetyltransferase (CAT) can acetylate and inactivate chloramphenicol.
• Resistance is typically plasmid-mediated and may involve efflux pumps reducing intracellular antibiotic concentrations.

Antimicrobial Spectrum:

✔ Gram-Positive Bacteria (Staphylococcus spp., Streptococcus spp.)

✔ Gram-Negative Bacteria (Escherichia coli, Salmonella spp., Klebsiella spp.)

✔ Mycoplasma & Mycobacteria

✔ Used in Veterinary Medicine & Ophthalmic Applications

Applications

🔬 Plant Cell Culture & Genetic Transformation – Used as a selection agent for transformed cells with chloramphenicol resistance genes

🦠 Microbial Pathogenesis & Antibiotic Resistance Studies – Supports bacterial inhibition research

⚗️ Molecular Biology & Cloning – Used in bacterial selection for plasmid maintenance (10–20 µg/mL)

🧪 Protein Expression & Recombinant DNA Technology – Ensures plasmid stability in E. coli cultures

🏭 Biopharmaceutical & Drug Development – Used in screening for new antibiotic formulations

Preparation & Storage Guidelines

1️⃣ Preparation of Stock Solution

• Dissolve 50 mg/mL in ethanol, methanol, or DMSO for best solubility.
• Aqueous solutions are neutral and stable over a wide pH range.
• Filter sterilization is recommended (do not autoclave).

2️⃣ Recommended Working Concentrations

• Bacterial Selection in Molecular Biology: 10–20 µg/mL
• Plant Cell Culture Transformation: 20–50 µg/mL

3️⃣ Storage Conditions

• Store at 2–8°C in a dry, well-ventilated place.
• Keep away from direct light to prevent degradation.

Ordering Information

Regulatory & Safety Information

⚠️ For Research Use Only (RUO). Not intended for clinical or therapeutic applications.

⚠️ Light-Sensitive & Hygroscopic – Store in a dry, well-ventilated environment.

⚠️ Use Protective Equipment (PPE) – Avoid direct skin contact & inhalation during handling.

Why Choose Sigma-Aldrich Chloramphenicol (BioReagent, Cell Culture Grade)?

🔹 High-Purity BioReagent (≥95%) for Research Applications

🔹 Broad-Spectrum Antibiotic Effective Against Gram-Positive & Gram-Negative Bacteria

🔹 Validated for Plant Cell Culture & Bacterial Selection (10–20 µg/mL)

🔹 Long-Term Stability Under Controlled Storage Conditions

🔹 Available in Multiple Pack Sizes for Research Flexibility

For bulk orders, technical support, or additional information, contact LabMart Limited today. 🚀