Sigma-Aldrich 3,3′,5,5′-Tetramethylbenzidine (TMB) Liquid Substrate System

The 3,3′,5,5′-Tetramethylbenzidine (TMB) Peroxidase Substrate by Sigma-Aldrich is a chromogenic liquid substrate optimized for ELISA (microwell) applications. It produces a blue reaction product upon oxidation by horseradish peroxidase (HRP), which transitions to yellow upon acidification with sulfuric acid.

Key Features:

1. Colorimetric Detection:
   • Blue end product: Measurable at 370 nm or 655 nm.
   • Yellow product after acid stop: Measurable at 450 nm.
2. Application Focus:
   • Specifically designed for ELISA (microwell) procedures.
   • Not recommended for membrane-based applications.
3. Ready-to-Use Format:
   • Simplifies workflows by eliminating preparation steps.
4. High Sensitivity:
   • Provides reliable signal generation for HRP-linked assays.
5. Light Sensitivity:
   • Requires protection from direct sunlight and UV sources to maintain stability.

Specifications:

Applications:

1. ELISA (Microwell Procedures):
   • Detects HRP-linked antibodies or proteins in a colorimetric assay format.
2. Research Studies:
   • Used in extending measuring ranges of immunoenzymometric assays.
   • Applied in studies of peroxidase-like activity in biomaterials (e.g., CuS concave superstructures).

Advantages:

1. Optimized for ELISA:
   • Delivers high signal-to-noise ratio in microwell assays.
2. Easy to Use:
   • Ready-to-use formulation eliminates preparation errors.
3. Reliable Results:
   • Provides consistent, reproducible results across experiments.

Cautions and Handling:

1. Light Sensitivity:
   • Store protected from direct sunlight or UV sources to avoid degradation.
2. Stability:
   • Ensure proper handling to maintain substrate activity and colorimetric performance.

Comparison with Similar Substrates:

Related Notes:

• Award Recognition: Winner of the 2024 CiteAb Award for contributions to Parkinson's research.
• Research Use Only: Not for diagnostic or therapeutic applications.