Sigma-Aldrich Stop Reagent for TMB Substrate powder, ELISA, 450 nm

The Stop Reagent for TMB Substrate is a specialized reagent for terminating the enzymatic reaction of TMB (3,3′,5,5′-Tetramethylbenzidine) in ELISA applications. It effectively stops the reaction and shifts the absorbance from blue (620 nm) to yellow (450 nm), allowing for accurate and consistent quantification of results.

Key Features:

1. Reaction Termination:
   • Rapidly halts the HRP-TMB reaction, preventing further color development.
   • Converts the blue reaction product (620 nm) to a stable yellow product measurable at 450 nm.
2. Specific Application:
   • Designed for use with TMB substrates in ELISA assays.
3. User-Friendly:
   • Provided in a powder format for flexibility in reagent preparation.
   • Ensures consistent and reproducible results.
4. Versatile Uses:
   • Applicable in various ELISA-based research studies.

Applications:

• In Vitro NETs Analysis:
  • Used to prepare and analyze neutrophil extracellular traps (NETs).
• ELISA Assays:
  • Direct binding ELISA to measure specific antigen-antibody interactions.
• Interference Assays:
  • Evaluates potential interference in biochemical reactions.

Specifications:

Advantages:

1. Enhanced Stability:
   • Ensures the yellow reaction product is stable for subsequent readings.
2. Precise Measurement:
   • Produces clear, consistent absorbance values for ELISA quantification.
3. Flexible Preparation:
   • Powder format allows for preparation of customized concentrations.

Comparison with Other Stop Reagents:

References for Use:

1. Preparation and analysis of neutrophil extracellular traps (NETs) in vitro.
2. Direct binding enzyme-linked immunosorbent assay (ELISA).
3. Use in interference assay studies.

Handling and Storage:

• Storage Conditions: Keep the powder reagent in a cool, dry place.
• Preparation: Reconstitute according to protocol for desired use.