Thermo Scientific™ Invitrogen™ Proteinase K (E.C.3.4.21.64)

The Invitrogen™ Proteinase K Recombinant Protein is a highly purified, recombinant enzyme derived from the P. pastoris expression system. This extracellular serine endoproteinase exhibits strong activity for the digestion of proteins and is widely used for protein and nucleic acid extraction. The enzyme is effective at high temperatures and wide pH ranges, making it ideal for various laboratory applications such as genomic DNA extraction, RNA extraction, and protein degradation.

The Proteinase K enzyme is a powerful protease known for its ability to break down proteins efficiently in the presence of detergents like SDS and urea, which are often used in cell lysis protocols. Additionally, Proteinase K is free from DNase and RNase contamination, making it suitable for use in nucleic acid-based procedures.

Key Features:

1. High Purity:
   • The enzyme is >95% pure as determined by SDS-PAGE, ensuring high reliability and performance in all applications.
2. Recombinant Protein:
   • Proteinase K is produced in the P. pastoris expression system, ensuring high consistency and animal-origin-free production.
3. Protease Activity:
   • >30 U/mg activity with casein as a substrate, ensuring efficient digestion of proteins at 37°C.
4. Wide Activity Range:
   • Active across a wide range of temperatures (optimal between 20-60°C) and pH (between 7.5-12.0), making it suitable for various sample types.
5. Effect of Detergents and Urea:
   • Activity is stimulated by up to 2% SDS or up to 4 M urea, making it suitable for dealing with protein denaturation or complex biological samples.
6. Calcium-Dependent Thermostability:
   • While calcium is not required for catalytic activity, it enhances thermostability, allowing the enzyme to function well at higher temperatures.
7. Non-DNase/RNase Contamination:
   • The enzyme is free from DNase and RNase contamination, as verified by digesting lambda DNA at 37°C for 6 hours and 25°C for 16 hours, making it ideal for nucleic acid work.
8. Lyophilized for Stability:
   • The enzyme is lyophilized for long-term stability, and once reconstituted, it is stable at -20°C for up to 1 year.
9. Storage Instructions:
   • Store lyophilized protein at 4°C for up to 1 year, and for long-term storage, keep at -20°C. Once reconstituted, store at 2–8°C for up to 6 months.

Specifications:

Applications:

1. DNA and RNA Extraction:
   • Widely used for proteinase digestion during nucleic acid extraction from tissues, cells, and biological samples.
2. Protein Degradation:
   • Degrades proteins in complex samples such as cell lysates, making it essential in cell biology and biochemistry studies.
3. Molecular Biology:
   • Used in the preparation of genomic DNA and RNA for PCR, gel electrophoresis, and sequencing.
4. Protein Purification:
   • Helps in protein purification by removing unwanted proteins that could interfere with subsequent analyses or assays.
5. Enzyme-Linked Assays:
   • Employed in enzyme assays where efficient protein degradation is necessary for accurate quantification.

Related Products:

• Proteinase K 20 mg/mL Solution
• Thermo Scientific™ DNA Extraction Kits
• Thermo Scientific™ RNA Isolation Kits
• Thermo Scientific™ Protein Lysis Buffers

Target Information:

Proteinase K is a serine protease that catalyzes the cleavage of proteins by breaking peptide bonds. It is highly effective in protein degradation and nucleic acid preparation. The enzyme is typically used to lyse cells, remove contaminating proteins, and prepare samples for molecular applications like PCR and DNA/RNA sequencing.

The Invitrogen™ Proteinase K Recombinant Protein is a highly reliable tool for protein degradation, DNA extraction, and biochemical research. Its broad temperature and pH stability, combined with its high activity, makes it ideal for various cellular and molecular biology applications. With its lyophilized form and long-term storage capabilities, it offers excellent flexibility and performance for a wide range of laboratory protocols.