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Specifications:
| Application | ELISA-Based Assays | ||
| Storage Temperature | 2-8°C | ||
| Product Type | Elisa Kit | Forms | Kit with Various components |
| Product Grade | Molecular Biology | ||
Quantitative Sandwich ELISA for Detection of Rat Tumor Necrosis Factor Alpha (TNF-α) in Serum, Plasma, and Cell Culture Supernatants
The Rat TNF-α ELISA Kit (EL0013Ra) is a high-sensitivity, quantitative sandwich ELISA designed for the accurate detection of tumor necrosis factor-alpha (TNF-α) concentrations in rat serum, plasma, cell culture supernatants, and other biological fluids. TNF-α is a pro-inflammatory cytokine primarily secreted by activated macrophages and plays a critical role in inflammation, immune response, and cellular signaling.
This assay uses HRP-conjugated streptavidin and a biotinylated detection antibody for signal amplification, with TMB as the substrate, offering a sensitivity of 0.43 pg/mL and a working assay range of 4.69–300 pg/mL.
Key Features & Benefits
- ✅ High Sensitivity (0.43 pg/mL) – Detect low-level expression in various matrices
- ✅ Wide Dynamic Range (4.69–300 pg/mL) – Suitable for physiological and pathological studies
- ✅ Validated for Multiple Matrices – Compatible with serum, plasma, and cell culture supernatants
- ✅ Strong Specificity – No cross-reactivity with related cytokines (IL-1β, IL-6, IFN-γ, etc.)
- ✅ Reliable Precision – Intra-assay CV <6.3%; Inter-assay CV <8.4%
- ✅ Comprehensive Kit – Includes standards, reagents, and pre-coated 96-well strip plate
- ✅ Storage at 2–8 °C – Stable for up to 2 years under proper conditions
- ✅ Research Use Only (RUO) – Designed for preclinical and laboratory applications
Kit Contents (96 Tests)
| Component | Volume/Quantity |
|---|---|
| Pre-coated 96-well plate | 1 (12 × 8-well strips) |
| Recombinant Rat TNF-α Standard | 2 vials (lyophilized) |
| Biotinylated Detection Antibody | 80 µL (100× concentrate) |
| Streptavidin-HRP | 150 µL (100× concentrate) |
| Standard Diluent | 5 mL |
| Assay Buffer (10×) | 5 mL |
| Wash Buffer (20×) | 50 mL |
| Substrate Solution (TMB) | 15 mL |
| Stop Solution (H₂SO₄, 0.18M) | 15 mL |
| Plate Sealers | 5 sheets |
| User Manual | 1 |
Performance Specifications
| Attribute | Value |
|---|---|
| Sensitivity | 0.43 pg/mL |
| Assay Range | 4.69 – 300 pg/mL |
| Intra-assay CV | <6.3% |
| Inter-assay CV | <8.4% |
| Recovery | 82–116% (mean = 96%) |
| Linearity (dilution) | 95–119% recovery across dilutions |
| Storage | 2–8 °C |
| Shelf Life | 2 years |
| Regulatory Status | RUO – Research Use Only |
Sample Types
- ✅ Rat serum
- ✅ Rat plasma (EDTA, citrate, or heparin)
- ✅ Cell culture supernatants
- ✅ Other biological fluids (e.g., cerebrospinal fluid, peritoneal fluid)
Applications
🧪 Inflammation and Immunology Research
🧬 Cytokine Quantification in Preclinical Studies
🔬 Drug Efficacy Evaluation in In Vivo Rat Models
📈 Biomarker Profiling in Transplantation and Autoimmunity
🧫 Cell Line Characterization and Cytokine Release Assays
The Rat TNF-α ELISA Kit (EL0013Ra) offers a robust and precise solution for quantitative cytokine analysis in rat research models. With high sensitivity, specificity, and reproducibility, this kit supports applications ranging from inflammation monitoring to drug response evaluation in preclinical and translational research.
- Pack Size: For 48 Tests For 96 Tests