Thermo Scientific™ RapID™ ONE System

Rapid 4-Hour Identification of Oxidase-Negative, Gram-Negative Bacilli

The Thermo Scientific™ RapID™ ONE System is a rapid enzyme-based identification system for the qualitative identification of over 70 medically important oxidase-negative, Gram-negative bacilli, including members of the Enterobacteriaceae family, from human clinical specimens.

Designed for simplicity and speed, the RapID ONE System uses chromogenic and conventional enzyme substrates in a one-step manual format that delivers accurate identification results in as little as four hours, improving turnaround times for critical microbiology workflows.

Key Features & Benefits

• Fast Results
  • Delivers definitive identifications in just four hours
  • RapID SS/u variant identifies some organisms in only two hours
• Simple Workflow
  • One-step inoculation—no pipetting, oil, or complex procedures
  • No need for 24-hour incubation or additional materials
• Compact & Efficient Format
  • Self-contained reagent-impregnated wells in a clear plastic tray
  • Allows simultaneous inoculation of all test cavities
  • Reduces consumable usage and preparation time
• Visible Color Changes
  • Provides easy-to-interpret visual results with minimal subjectivity
  • Some reactions require reagent addition to complete interpretation
• Broad Coverage with High Accuracy
  • Identifies a wide range of oxidase-negative, Gram-negative bacilli, including:
    • E. coli
    • Klebsiella spp.
    • Enterobacter spp.
    • Proteus, Citrobacter, Serratia, and others
• Data-Driven Reporting
  • Identification is based on pattern recognition using
    • RapID™ Code Compendium (ERIC) or
    • Software-based reactivity databases

Intended Use

The RapID ONE System is intended for the qualitative in vitro identification of oxidase-negative, Gram-negative bacilli, especially members of the Enterobacteriaceae family, from colonies grown on solid media. It is designed for use in clinical microbiology laboratories to support diagnosis and treatment decisions.

For professional use only. Not for use with mixed cultures or direct specimen testing.

Ordering Information

Each kit includes 20 test panels and required reaction wells.

Reagents not included—must be ordered separately.

Required Accessories (Sold Separately)

Specifications

Workflow Overview

1. Prepare bacterial suspension in RapID Inoculation Fluid
2. Inoculate test panel using one-step method
3. Incubate at 35–37°C for 4 hours
4. Observe color changes in wells
5. Add reagents to specific wells (if required)
6. Record positive/negative reactions
7. Compare reaction pattern with ERIC database or Code Compendium to identify isolate

Precautions

• For in vitro diagnostic use
• Use only by trained personnel
• Do not use if packaging is damaged or expired
• Requires pure isolates for accurate identification
• Results should be interpreted in context of other biochemical and clinical data

The Thermo Scientific™ RapID™ ONE System offers rapid, reliable, and cost-effective identification of oxidase-negative, Gram-negative bacteria—dramatically improving time-to-result compared to conventional biochemical tests. With its simple format, short incubation, and broad organism coverage, it is a trusted solution for clinical microbiology laboratories focused on efficiency and accuracy in pathogen identification.