SensiMix™ SYBR® Low-ROX Kit

The SensiMix™ SYBR® Low-ROX Kit has been developed for highly accurate real-time PCR and has been validated on real-time PCR platforms that require low concentrations of the passive reference dye ROX for normalization of well-to-well differences.

A proprietary, highly-optimized, chemical hot-start PCR enzyme promotes highly-specific amplification, in turn improving assay sensitivity and dynamic range, ensuring that SensiMix SYBR® Low-ROX Kit provide the sensitivity and specificity required for demanding assays under standard thermal cycling conditions. 

The SensiMix SYBR® Low-ROX Kit has been optimized to deliver optimal performance in tandem with the SensiFAST cDNA Synthesis Kit, which offers fast, unbiased cDNA synthesis, without compromising cDNA yield or coverage.

• Sensitive - reproducible detection of low-copy number templates
• Reproducible – consistent results between technical replicates for increased confidence in results
• Specific - proprietary hot-start modification minimizes non-specific amplification for improved assay reliability in high-throughput assays
• Robust – reliable, accurate detection of DNA and cDNA targets from a broad range of sample types
• Applications: Gene expression analysis, DNA / cDNA target detection, miRNA profiling / quantification, copy number variation (CNV) analysis
• For ease-of-use and added convenience, SensiMix SYBR® Low-ROX is provided as a 2x mastermix containing all the components necessary for real-time PCR, including the SYBR® Green I dye, dNTPs, stabilisers and ROX for optional use
• QT62505: 500 x 50 µL Reactions: 10 x 1.25 mL
• Storage: -20°C upon receipt for optimum stability, repeated freeze/thaw cycles should be avoided, avoid exposure of the SYBR® Green I to light
• Stability: When stored under the recommended conditions and handled correctly, full activity of the kit is retained until the expiry date indicated on the outer box label