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Specifications:
| Application | Cell Analysis | ||
| Storage Temperature | -15°C to -25°C | ||
| Product Type | Assay Kits | Forms | Liquid |
| Product Brand | Thermo Fisher Scientific™ | ||
| Product Grade | Molecular Biology | ||
The Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit provides a bright, stable, and long-lasting bioluminescent signal (half-life > 2 hours) for quantitative detection of firefly luciferase activity in mammalian cell lysates. Ideal for promoter activity studies, drug screening, RNA interference assays, and multiplexed reporter studies, this kit offers exceptional signal stability without requiring a luminometer with injectors.
By using D-luciferin as a substrate, the glow assay delivers sustained light emission, enabling high-throughput and batch processing while maintaining accuracy and reproducibility.
Key Features
- Highly Sensitive – Detects low levels of firefly luciferase activity with excellent signal-to-noise ratio.
- Extended Signal Stability – Half-life greater than 2 hours, eliminating the need for fast injector systems.
- Simple Workflow – Ready-to-use reagents for rapid assay setup.
- Automation-Friendly – Compatible with 96- and 384-well formats for high-throughput applications.
- Compatible with Enhancers – Use with Pierce™ Firefly Luciferase Signal Enhancer for maximum brightness.
- Safe & Non-Radioactive – Eliminates hazards associated with radioactive assays.
Applications
- Promoter Studies – Analyze cis-regulatory elements and trans-acting factors.
- Drug Screening – Identify compounds affecting gene expression.
- siRNA & miRNA Screening – Assess gene silencing efficiency.
- Multiplex Reporter Assays – Study off-target effects in combination with other luciferase reporters.
- Protein Localization Studies – Track protein expression via luciferase tags.
- Signal Transduction Pathway Analysis – Evaluate pathway activation or inhibition.
- RNA Splicing Studies – Investigate splicing events linked to luciferase expression.
Technical Specifications
| Parameter | Specification |
|---|---|
| Detection Method | Bioluminescence |
| Target | Firefly Luciferase |
| Substrate | D-Luciferin |
| Signal Stability | > 2 hours |
| Format | 96-well or 384-well plates |
| Compatible Cells | Mammalian cells |
| Technique | Enhanced Chemiluminescence |
| Kit Sizes | 100 reactions (Cat. No. 16176), 1000 reactions (Cat. No. 16177) |
| For Use With | Luminometer (microplate) |
| Storage | –20°C (buffer), 4°C (luciferin), RT (lysis buffer) |
Kit Contents
- Firefly Glow Assay Buffer – 5 mL (store at –20°C)
- D-Luciferin (Lyophilized) – 3 mg (store at 4°C)
- 2X Cell Lysis Buffer – 6 mL (store at room temperature)
The Pierce™ Firefly Luciferase Glow Assay Kit delivers exceptional performance for luciferase reporter assays with unmatched signal stability, making it ideal for batch processing, high-throughput screening, and long-term kinetic studies. Whether for gene expression analysis or drug discovery, it ensures reliable and reproducible results in any luminescence-capable setup.
- Reactions: 100 rxn 1000 rxn