MinElute PCR Purification Kit

Features

• Very small elution volumes
• Fast procedure and easy handling
• High, reproducible recoveries
• Gel loading dye for convenient sample analysis

Product Details

The MinElute PCR Purification Kit provides spin columns, buffers, and collection tubes for silica-membrane-based purification of PCR products 70 bp – 4 kb in size. The spin columns are designed to allow elution in very small volumes (as little as 10 μl), delivering high yields of highly concentrated DNA. An optional pH indicator allows easy determination of the optimal pH for DNA binding to the spin column. The procedure can be fully automated on the QIAcube Connect.

Performance

The MinElute PCR Purification procedure removes primers, nucleotides, enzymes, mineral oil, salts, and other impurities from DNA samples (see figure " Efficient primer removal").

The MinElute PCR Purification Kit provides spin columns for PCR product cleanup. Using a microcentrifuge or vacuum manifold, high concentration of DNA fragment (70 bp – 4 kb) is quickly achieved. (DNA fragments larger than 4 kb should be purified using the QIAquick PCR Purification Kit.)

See figures

Efficient primer removal.

Principle

The MinElute PCR Purification Kit contains a silica membrane assembly for binding of DNA in high-salt buffer and elution with low-salt buffer or water. Silica-membrane technology eliminates the problems and inconvenience associated with loose resins and slurries. 

Gel loading dye

To enable faster and more convenient sample processing and analysis, gel loading dye is provided. GelPilot Loading Dye contains three tracking dyes (xylene cyanol, bromophenol blue, and orange G) to facilitate the optimization of agarose gel run time and prevent smaller DNA fragments migrating too far (see figure " GelPilot Loading Dye").

See figures

GelPilot Loading Dye.

Procedure

The MinElute System uses a simple bind-wash-elute procedure. Binding buffer is added directly to the PCR sample or other enzymatic reaction, and the mixture is applied to the MinElute spin column. The binding buffer contains a pH indicator, allowing easy determination of the optimal pH for DNA binding (see figure  "pH Indicator Dye"). Nucleic acids adsorb to the silica membrane in the high-salt conditions provided by the buffer. Impurities are washed away and pure DNA is eluted with a small volume of low-salt buffer provided or water, ready to use in subsequent applications.

Handling

MinElute spin columns are designed to provide two convenient handling options (see flowchart "MinElute procedure"). The spin columns fit into a conventional table-top microcentrifuge or onto any vacuum manifold with luer connectors, such as QIAvac 24 Plus and can also be fully automated on the QIAcube Connect (see figures "Spin column handling options  A and  B" and " QIAcube Connect").

See figures

pH Indicator Dye.Spin column handling options — A.

Spin column handling options — B.QIAcube Connect.

Applications

DNA fragments purified with the MinElute System are ready for direct use in all applications, including:

• Sequencing, including next generation sequencing
• Microarray analysis
• Ligation and transformation
• Restriction digestion
• Labeling

Specifications