MC1568 99.51%

MC1568 is a selective histone deacetylase inhibitor with activity against class IIa HDACs and reported inhibitory activity against maize HD1-A. In cell-free assays, MC1568 inhibits maize HD1-A with an IC50 of 100 nM and shows approximately 34-fold selectivity for HD1-A over HD1-B.

MC1568 is widely used in epigenetics, differentiation, myogenesis, adipogenesis, transcriptional regulation, and HDAC signalling research. It has been studied in several cellular models, including breast cancer cells, myoblasts, embryonal carcinoma cells, adipocyte differentiation models, and mouse tissue-selective HDAC inhibition studies.

Key Features

• Selective HDAC inhibitor with class IIa HDAC activity
• Inhibits maize HD1-A with IC50 of 100 nM
• Approximately 34-fold selectivity for HD1-A over HD1-B
• Reported class II HDAC IC50 of 220 nM
• Demonstrates strong selectivity for class II HDACs over class I HDACs
• Inhibits HDAC4 activity in human breast cancer cell lysates
• Used to study myogenesis, adipogenesis, endodermal differentiation, and PPARγ signalling
• Suitable for epigenetics, chromatin regulation, differentiation, and transcriptional research
• Commonly prepared in DMSO for in vitro studies
• For research use only

Target Profile

Biological Activity

MC1568 has been studied as a selective class IIa HDAC inhibitor in both cell-free and cell-based models. In human breast cancer ZR-75.1 cell lysates, MC1568 at 5 µM showed no inhibitory activity against HDAC1 but inhibited HDAC4, supporting its class II HDAC selectivity.

In MCF-7 cells, MC1568 at 20 µM increased the accumulation of acetylated histone H3 and H4, as well as acetylated tubulin, indicating effects on histone acetylation and HDAC6-associated pathways.

In C2C12 myoblasts, MC1568 at 5 µM has been reported to arrest myogenesis by reducing MEF2D expression, stabilizing the HDAC4-HDAC3-MEF2D complex, and inhibiting differentiation-associated MEF2D acetylation.

In Vitro Research Applications

Cell-Based Research Data

HDAC Enzyme Inhibition Assay Summary

MC1568 has been evaluated in maize HDAC enzyme inhibition assays using a radiolabelled histone substrate method. The assay measures the release of tritiated acetic acid from prelabelled histones and quantifies activity by scintillation counting.

In Vivo Research Summary

MC1568 has been studied in mouse models for tissue-selective HDAC inhibition and transcriptional signalling research. In mice, MC1568 at 50 mg/kg has shown apparent tissue-selective HDAC inhibition, particularly in skeletal muscle and heart.

Research Findings Summary

Technical Summary

Storage and Handling Notes

MC1568 should be handled as a research chemical by trained laboratory personnel using appropriate chemical safety procedures.

Recommended handling considerations include:

• Store according to supplier recommendations
• Prepare stock solutions using a suitable solvent such as DMSO
• Aliquot prepared stock solutions to avoid repeated freeze-thaw cycles
• Protect working solutions from unnecessary light and moisture exposure
• Use appropriate PPE, including gloves, lab coat, and eye protection
• Dispose of chemical waste according to institutional safety procedures

MC1568 is a selective HDAC inhibitor used for studying class IIa HDAC biology, maize HD1-A inhibition, chromatin regulation, myogenesis, adipogenesis, nuclear receptor signalling, and tissue-selective HDAC activity. Its reported selectivity for class II HDACs, activity against HDAC4-related pathways, and effects on MEF2D and PPARγ-associated differentiation make it a useful research compound for epigenetics, developmental biology, and cellular differentiation studies.