Human Aflatoxin Albumin Adduct (AFT-HSA) ELISA Kit

The Human Aflatoxin Albumin Adduct (AFT-HSA) ELISA Kit (MBS3802734) is a high-quality, ready-to-use microwell strip plate assay designed for the quantitative detection of Aflatoxin Albumin Adduct (AFT-HSA) in biological samples. This kit leverages the enzyme-linked immunosorbent assay (ELISA) method, utilizing AFT-HSA antibody-antigen interactions and an HRP colorimetric detection system for accurate and sensitive analysis.

Key Features

1. Specificity:
   • Detects native AFT-HSA in biological samples, excluding recombinant forms.
2. Quantitative Analysis:
   • Includes calibration standards to generate a standard curve for precise measurement of AFT-HSA concentration.
3. Wide Sample Compatibility:
   • Suitable for undiluted body fluids, tissue homogenates, and secretions.
4. Reproducibility:
   • Demonstrates reliable intra-assay CV (%) and inter-assay CV (%) for consistent results.
5. Ease of Use:
   • Ready-to-use components simplify setup and streamline the workflow.

Test Principle

The assay is based on the immunosorbency principle, utilizing AFT-HSA antibody-antigen interactions, and includes the following steps:

1. Pre-Coated Plate:
   • The microtiter plate is pre-coated with an antibody specific to AFT-HSA.
2. Sample and Standard Addition:
   • Standards and samples are added to the wells, where AFT-HSA antigens bind to the pre-coated antibody.
3. HRP-Conjugated Detection:
   • An HRP-conjugated detection antibody is added, binding to the antigen-antibody complex.
4. Colorimetric Reaction:
   • A TMB substrate solution reacts with HRP, producing a blue color.
5. Stop Reaction and OD Measurement:
   • The reaction is terminated, turning the color yellow. The optical density (OD) is measured at 450 nm using a spectrophotometer.
6. Concentration Determination:
   • AFT-HSA concentrations are determined by comparing OD values to the standard curve.

Specifications

Applications

• Toxicology Research:
  • Assess exposure to aflatoxins through the detection of AFT-HSA.
• Public Health Studies:
  • Monitor aflatoxin biomarkers in populations at risk.
• Food Safety Research:
  • Evaluate the biological effects of aflatoxin contamination.
• Environmental Studies:
  • Investigate aflatoxin exposure in animals and humans.

Kit Components

• Pre-coated microtiter plate.
• AFT-HSA calibration standards.
• HRP-conjugated detection antibody.
• TMB substrate solution.
• Stop solution.
• Wash buffer.
• Sample diluent.
• Instruction manual.

Advantages

1. High Sensitivity:
   • Accurately detects low concentrations of AFT-HSA in samples.
2. Reliable Reproducibility:
   • Consistent results across and within assays.
3. Wide Sample Utility:
   • Compatible with diverse biological sample types.
4. Ease of Calibration:
   • Calibration standards ensure precise quantification.
5. Streamlined Workflow:
   • Ready-to-use components minimize preparation time.

Storage and Stability

• Store kit components as recommended in the provided instructions.
• Avoid repeated freeze-thaw cycles to maintain reagent integrity.

The Human Aflatoxin Albumin Adduct (AFT-HSA) ELISA Kit provides a robust, reliable, and sensitive solution for detecting and quantifying AFT-HSA in various biological samples. Its specificity, reproducibility, and straightforward workflow make it an invaluable tool for toxicology research, public health studies, and environmental monitoring.