Mabtech Anti-human IFN-γ mAb (1-D1K), unconjugated

The Mabtech Anti-human IFN-γ mAb (1-D1K) is a monoclonal antibody designed for the specific detection of human IFN-γ in a variety of immunoassays, including ELISpot, FluoroSpot, ELISA, and Immunocytochemistry. This antibody is unconjugated, providing flexibility for customized labeling or use in combination with other reagents. It is ideal for cell-based assays and quantification of IFN-γ in human samples.

Key Features:

1. Specific Detection of Human IFN-γ:
   • 1-D1K monoclonal antibody is highly specific for native and recombinant human IFN-γ, enabling accurate detection and quantification in immunoassays.
2. Versatile Applications:
   • Suitable for use in ELISpot, FluoroSpot, ELISA, and immunocytochemistry, making it ideal for cellular immunoassays and protein detection in human samples.
3. Purified by Protein G Affinity Chromatography:
   • The antibody is purified from in vitro cultures using Protein G affinity chromatography, ensuring high specificity and purity.
4. Flexible Detection Options:
   • Unconjugated antibody allows custom labeling or use in combination with other detection reagents, providing flexibility in experimental design.
5. High Concentration:
   • Supplied at 1 mg/mL, making it suitable for a wide range of applications requiring high concentrations of the antibody.
6. Storage and Stability:
   • Stable at 4-8°C short term and -20°C long term. The antibody has a shelf life of 18 months from the date of receipt when stored correctly.
7. Recombinant Immunogen:
   • Immunogen used for 1-D1K antibody development is recombinant human IFN-γ, ensuring the antibody’s high specificity for the antigen.

Specifications:

Applications:

1. ELISpot and FluoroSpot Assays:
   • The 1-D1K antibody is recommended as a capture antibody in ELISpot and FluoroSpot assays for quantifying IFN-γ secretion by immune cells, especially T-cells.
2. ELISA for Cytokine Detection:
   • Ideal for detection and quantification of IFN-γ in ELISA formats, facilitating immune response studies and immunology research.
3. Immunocytochemistry (ICC/IF):
   • Visualize IFN-γ expression in cell cultures under fluorescent or light microscopy, important for apoptosis studies and cytokine profiling.
4. Protein Detection in Apoptosis and Immunology:
   • Caspase 3 and IFN-γ signaling pathways are studied using this antibody, essential for immune research and infectious disease studies.
5. Flow Cytometry:
   • Useful in flow cytometry applications for quantifying IFN-γ production by immune cells in response to specific stimuli.

IFN-γ (Interferon gamma) is a cytokine produced by T-cells and natural killer cells, playing a central role in immune responses. It is involved in immune cell activation, apoptosis, and inflammation. Caspase 3 activation and IFN-γ secretion are critical markers in immune modulation, particularly during immune responses to pathogens, viruses, and cancer cells. The 1-D1K antibody specifically detects IFN-γ to study immune system function and cytokine production in cell-based assays.